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We narrowed to 1,034 results for: LAS

Showing: 261 - 280 of 1034 results

  1. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9-Cas9 ...Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants...post Read the paper in Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control...paper in Nature Methods Find the anti-CRISPR plasmids Tagging human transcription factors with GFP...White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors with GFP. As...

  2. Five Popular Model Organisms, Part 2

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    Blog Post
    ...our plasmid collection page Find more plasmids and resources for plant resources on Plant Plasmids Collection...box” for its common use in plasmid manipulation and genetics research. Plasmids are ideal tools for studying...Model Organisms blog post Read more about plasmids in our Plasmids 101 blog series Learn more about CRISPR...disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl...several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced... and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from...transformations and the use of Arabidopsis in the classroom on our blog. Now that you’ve learned more about...

  3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ... were in the cytoplasm of dendrites, and only a small proportion were found on the plasma membrane (top...to gold particles localized in the cytoplasm or bound to the plasma membrane. Note that the use of the... the DREADD receptors need to be located on the plasma membrane to be activated by the DREADD actuator...membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins Given... the limited expression of hM4Di‐mCherry at the plasma membrane in monkey neurons, we next hypothesized...might improve the transport of this DREADD to the plasma membrane. The haemagglutinin (HA) tag is a small...tag resulted in abundant expression of hM4Di in plasma membranes in both species (bottom panel in Fig....

  4. Addgene's Top Blog Posts from 2020

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    Blog Post
    ...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...CRISPR to detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without...

  5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ... target a cell class in mice is not a good predictor for targeting the same cell class in NHPs (R=0.38... target a cell class in monkeys was a good predictor for targeting the same cell class in humans (R=0.67... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...specific cell types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods...targeting More than 70% of expression was from a single class of cells  Of the four different promoter designs...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...the odds of that promoter targeting the same cell class in all three species are still greater than random...

  6. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post
    ... in E. coli with two plasmids (Figure 3 below): Input Plasmid: The input plasmid (high copy, ColE1 origin...inducible promoter (PPhlF).  Output Plasmid: The output plasmid (single copy, bacterial artificial chromosome...through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All two-input...indicated figures in our paper (1)) Input Plasmid Output Plasmid Fig. 3A pNR64 pNR160 Fig. 4A pNR220...Materials and Methods section of our paper (1). Lastly, it is possible to build gene regulatory RSMs (...two-input RSMs and three three-input RSMs. The plasmids used to construct these RSMs are listed in the...paper (1). These parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We ...

  7. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...Baird, who has created plasmids as a technician in the Davidson lab for the last four years. “Researchers...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...resources to go with them. This collection of plasmids is available in an easily searchable format on Addgene...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over...list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through...Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins...Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird has a helpful tip for anyone...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ... tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during the... human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside the ...) are aligned to the reference sequence of the plasmid used to create the viral prep. In a clean viral...this reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the...bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In...correspond to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database...database, and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

  9. Genome-wide Screening Using CRISPR

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    Blog Post
    ...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly... target every gene in the genome, or a specific class of genes? Addgene currently carries several genome-wide... selection of sub-libraries targeting specific classes of human genes. What are the steps involved in ...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...

  10. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...bacteria with the plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows... Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the...about Developing Lab Management Software    The plasmid's journey begins First, each package is opened and... to hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits...allow us to store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has ...strains that have been treated to easily take in plasmids (transformation). Transformed bacteria are then...

  11. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post
    ...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made...Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including ...antibodies that you have come to expect from our plasmid and viral services. The first step will be working...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...through our usual service (see our current antibody plasmid collection) and we plan to introduce affinity reagents...through distributions of research materials, such as plasmids and viral vectors. However, it typically takes...

  12. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post
    ...For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists...synthetic biology page Search for a plasmid Find Addgene's most popular plasmids ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...at its sequence. MIT collaboration for gene classification This inquiry started when Neil Thompson, a ...is synthetic or natural with 97.7% accuracy. Classifying genes as natural or synthetic But how can you...organism, it may express protein more efficiently. To classify gene sequences as natural or synthetic, the researchers...simulations and machine learning, they created a classifier that predicts whether a gene was natural or synthetic...

  13. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...pooled library, which contains a collection of plasmids in a single tube, or a pre-made viral prep library... prepare the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral...library and appropriate packaging and envelope plasmids into HEK cells allows production of lentivirus...amplify the library. This is similar to amplifying a plasmid: transform E. coli with the pooled library DNA,...own protocol that amplifies without disrupting plasmid representation. A good place to start is with a...

  14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post
    ...that the plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe... 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK... Fig. 1: Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to ...overexpressed compared to accessory proteins. Excess gvpA plasmid added into the transfection mixture in HEK293T ...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection... into the genome by cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience.... These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added...neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the...recombinase. After in-utero electroporation, this plasmid mix results in sparse labeling of cortical neurons...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...

  16. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ... After all, students build circuits in physics class to learn about electronics or mix chemicals together...everyone safe. None of this is conducive to a classroom setting, especially for a school with a limited... activities making their way into high school classrooms, like gel electrophoresis to separate DNA or ...translation. Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and ...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water ...developed the BioBits kit to be used in high school classrooms, but it could be widely adapted and used in a...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on...

  17. Reagent Repositories Are Speeding up Science During the Pandemic

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    Blog Post
    ...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents...

  18. Fluorescent Proteins 101: Luciferase

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    Blog Post
    ...Read More: All Plasmids 101 Articles Browse plasmids that contain luciferase ...Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting...choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert ...chemical reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the different...hirtus) 630 64 Luciferin ATP No Common uses for plasmids expressing luciferase Luciferase reporter assay...assay The typical luciferase-containing plasmid is most often used to investigate the effect of regulatory... cloned upstream of the luciferase gene in the plasmid, which is then introduced into the desired mammalian...

  19. Introducing Addgene's Microbiology Pages

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    Blog Post
    ...Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with fluorescent... To make it easier for you to find plasmids for your work, plasmids in the Microbiology Collection are... a useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally...us at [email protected]. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene...develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org/deposit ...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector... express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing... (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based ...been engineered to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction...own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...
Showing: 261 - 280 of 1034 results