Visualizing Genomic Loci with CRISPR-Sirius
Type
Blog Post
...needed to deliver CRISPR-Sirius into cells. They named this guide sgRNA-Sirius-8XMS2 which will recruit...
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Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...conjunction of vectors and exosome was given the name vexosomes (vector-exosomes), exosome AAVs, or exo-AAVs... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...clutter ofoutdoors-only spiders, all of whom are named Charlotte, all of whom are still working on theirpenmanship... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question must be... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...monitoring neuronal activity in the form of calcium dynamics. (For more details on this technology, see our... -
K. phaffii: Rising to the Occasion
TypeBlog Post... joy, but I think it’s acceptable if it’s in the name of beer and bread. The following table lists some... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...second group read the same abstract but with the names reversed, meaning that the findings of the study... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...studying infected lung tissue, a Danish biologist named Christian Gram realized that certain stains were... -
DAP arrays for multiplex gene editing
TypeBlog Post...release individual gRNAs for gene editing. They named this new array architecture the DAP array, short... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...photophysical properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...commonly used selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...downstream signaling cascades. So let’s break down the name "G-protein coupled receptor" into two parts: the... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...and CP1041-SpCas9. The resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed... -
A Deep Dive into BioBuilder
TypeBlog Post...in synthetic biology has grown into a recognized name in the field of STEM education worldwide. The BioBuilder... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...PMID: 27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization during ligand... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post... pLKO-Tet-On in the original publication but its name was subsequently changed to clarify that it does... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...previously worked at The Allen Institute for Neural Dynamics and HHMI’s Janelia Research Campus. His research... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...options. Table 2: Properties of luciferase enzymes Name Natural or engineered Peak emission (nm) Size (kDa... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...Addgene depositor Stephen Elledge. His new method, named sequence- and ligation-independent cloning (SLIC...