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We narrowed to 550 results for: NOL

Showing: 261 - 280 of 550 results
  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...you have any questions or thoughts about this technology, leave them in the comments below.  References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...
  2. Antibodies 101: Conjugation

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    Blog Post
    ...References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler...
  3. To Each HIS Own

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    Blog Post
    ...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...
  4. CRISPRainbow and Genome Visualization

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    Blog Post
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....
  5. Developing Lab Management Software for Biology

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    Blog Post
    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...running and more time working on interesting biotechnology code.There are valid technical reasons to choose...
  6. Transferable Skills Guide: Cross-team Communication

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    Blog Post
    ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...
  7. Viral Vectors 101: Virus Safety

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    Blog Post
    ...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...
  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post
    ...team will communicate with your organization’s technology transfer office or other authorized office directly... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...
  9. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...Cre is expressed.  Several variations of this technology can be used to customize the effects of this ...
  10. 6 Tips for Grant Writing

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    Blog Post
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...
  11. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...
  12. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...
Showing: 261 - 280 of 550 results