We narrowed to 568 results for: PAC;

1. Addgene's Top 10 Blog Posts of 2015

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   Blog Post

   ...for in vivo gene delivery, but have limited packaging capacity making them difficult to use with CRISPR ...

2. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

   ...plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number... discovery in the 1950s, plasmids have greatly impacted many areas of biological research and have been...

3. Don’t Wait To Launch Your Health Science Startup

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   Blog Post

   ...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

4. Another Pathway into Cells: iTOP

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   Blog Post

   ...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...directly with proteins removes the limitations of packaging size associated with viral vectors. For these ...

5. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   Blog Post

   ...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...

6. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   Blog Post

   ...FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions...biomarkers, and recently, cancer research related to space medicine. Now he aims on tracking pollution in rivers...

7. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...plasmid DNA that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks....

8. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

   ...can be limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...These loops not only provide a 3-D structure for packing genomic DNA into nuclei, but are also thought to...

9. Summer SciComm Series: A PhD in Science Communication

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   Blog Post

   ...menstrual pads while we talked about the environmental impact of disposable products. A great deal of scientific...Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived climate...

10. DAP arrays for multiplex gene editing

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    Blog Post

    ...promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo applications...therapeutic potential of the DAP array, the Gao Lab packaged MBE and MPE elements for deliveries via adeno-...

11. Clear and Accessible Writing

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    Blog Post

    ...the use of alt text to describe images in online spaces. Alt text is used by screen readers to provide ...professional development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.”...

12. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature...

13. Viral Vectors 101: Biosensors

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    Blog Post

    ...with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...AAV as your vehicle, we’ve got you covered with packaging plasmids! References and Resources References...

14. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...tricky to find: each of the factors can negatively impact both translation and fidelity of translation, leading.... Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan...

15. Viral Vectors 101: Viral Applications

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    Blog Post

    ... these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral applications...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...

16. AAV Q&A with Tim Miles

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    Blog Post

    ...why hot dogs are sold in packages of 8 yet hot dog buns are sold in packages of 10…)  If you missed the...

17. Hot Plasmids - February 2022

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    Blog Post

    ... of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple...significant off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor...

18. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ...of the antibody - the paratopes - may change and impact the specificity and avidity of the antibodies produced... chain or light chain genes within a hybridoma impacts the specificity, avidity, and number of different...

19. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ... about and develop technologies that will have impacts on many different facets of life. It also seems...neuroscience. AAV tools from Addgene are already having an impact on the field Not to toot our own horn too much,...

20. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post

    ... life cycles bacterial DNA can be accidentally packaged into the newly created phages. Transfer of this...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles with...