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We narrowed to 395 results for: POR C

Showing: 261 - 280 of 395 results

  1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs...back in with the Addgene blog for future award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima ...

  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...depositing can be grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...

  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...Antonini, M.-J., Rosenfeld, D., Park, J., Garwood, I. C., Yan, W., Niroui, F., Fink, Y., Chandrakasan, A.,...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this ...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...

  5. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  8. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... system is capable of robust expression of high G+C content genes, as validated through monitoring the...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...arrays (e.g. for spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... Find plasmids from this paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for ...

  11. Synthetic Biology - Overview

    Type
    Collection
    ...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver...GMP) FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-...Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010...Plasmids Joel Kralj Voltage Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...

  13. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...and be ready to provide extensive logistics support for export and shipping on dry ice. The Business and...seed money was used to build a completely self-supporting service so that Addgene is not dependent on grants... in accelerating the Viral Service. With their support we are able to provide faster access to a larger...number of viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing...Addgene repository. Without these scientists and the support of their technology transfer offices, Addgene would...

  14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...

  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

  17. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...    References and Resources References Drummond, C., & Fischhoff, B. (2017). Individuals with greater...how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight...to all the optional sexual health sessions and reported the intel back to my shyer classmates. For my ...the community During my PhD, I’ve also found opportunities for more hands-on science communication. Between...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    ... tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ... Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is not maintained. Recombination...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...ti2learning.org. References 1. Dieterich, Daniela C., et al. "In situ visualization and dynamics of newly...designated work areas and come with health risks. Importantly, with this method it is not possible to follow...azidohomoalanine (AHA). In the technique, AHA is incorporated into newly synthesized proteins instead of methionine...endogenous protein of interest that also had incorporated the AHA tag after pulse labeling of a determined...FUNCAT-PLA provides both quantitative and spatio-temporal information about the turnover of specific endogenous..."Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains." Proceedings...Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings of the National Academy...
Showing: 261 - 280 of 395 results