We narrowed to 353 results for: Plat

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...

2. 3D Printing Meets CRISPR Cas9

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   ...cleavage”. Jacob computationally added the non-template strand to the Jinek structure so that the resulting...

3. Truncated gRNAs for Regulating Gene Expression

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   ... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...

4. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...’s best to turn to an established social media platform. During the conference If the conference has a...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

6. Plasmids 101: Protein Expression

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   ...expression consists of transfecting cells with your DNA template of choice and allowing these cells to transcribe...

7. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   Blog Post

   ...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

11. Viral Vectors 101: The Retroviral Lifecycle

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    ...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....

12. CRISPR 101: RNA Editing with Cas13

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    ...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

14. MXS Chaining

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    ...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...

15. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

17. Isolating Genomic Regions of Interest with the CRISPR System

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    ...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...

18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

19. Negative Can Be Positive: Open AAV Data with Addgene

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    .... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...

20. Tips for Screening with Yeast Two Hybrid Systems

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    ... strain for screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  ...