We narrowed to 349 results for: Plat

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...

...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

.... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...

...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....

...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...

...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...

... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...

...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

... strain for screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  ...

...confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Comparing Cpf1...

...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...

...through the use of popular Chinese social media platforms.  As you can imagine, shipping to so many countries...

...chromosomal replication. Adapted from BioRender template. Since DnaA binding is necessary to initiate...

... also compatible with both AAV and adenovirus templates. The system, which is larger than Cas9, can also...

...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you...

... an intrinsically high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059...