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We narrowed to 583 results for: Ret;

Showing: 261 - 280 of 583 results

  1. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...

  3. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...

  4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  7. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ..., and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human T lymphocytes for...

  9. All Antibodies

    Type
    Collection
    ... Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for other antibodies...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  12. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  13. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  14. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curve, and most results can be confidently interpreted as integers. Since each sample is partitioned...method you choose, resist the temptation to over interpret your results: Sybr cannot be used to determine...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...

  18. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...
Showing: 261 - 280 of 583 results