... is to be successful, cancer immunotherapy must aspire to be a field of healthcare as dynamic as cancer...

...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed...

...panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation Laser Color Very Bright Bright...

...Reduced waste from kit materials Reduced plastic in spin columns and buffer bottles Waste tubes are made...

...postdoctoral training from Massachusetts General Hospital and Harvard Medical School. Connect with her on...

...quarters of 2017. As an international researcher and aspiring entrepreneur, you have many career options available...

...party goers, the piñata bursts and its contents spill to the ground. There is something akin to a frenzy...

...1 Gp95, NT3 SPP1 secreted phosphoprotein 1 BNSP, BSPI, ETA-1, MGC110940, OPN SST somatostatin SMST SSTR1...

...induction of structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene...

...containing a C nucleotide at the same position in spite of being a perfect match for the target sequence...

...for spatial proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation...

... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...

...livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan...

... Harvest Gently aspirate media from the 6-well plates. Be sure to use a new aspirating pipette for each... 37 °C for 30 min. Gently aspirate media from wells, using a new aspirating pipette for each well. Wash...Wash wells with 1 mL of 1X PBS and aspirate, using a new aspirating pipette for each well. Detach cells...Equipment Class II, Type A2 Biological Safety Cabinet Aspirating unit Microcentrifuge, Eppendorf, 5425R Droplet...tube. Centrifuge for 5 min at 100 x g . Gently aspirate supernatant. Wash cell pellets in 500 µL PBS. .... Centrifuge for 5 min at 100 x g . Gently aspirate supernatant. Extract genomic DNA according to the ...primers/probe mixes and master mix for 15 sec then spin 10 sec in a mini centrifuge and place on ice. Wipe...

...plate seal from the 96-well plate and set aside. Aspirate the wells and use a multichannel pipette to add...a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare the blocking...a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare antibody...a total of three washes. After the final wash, aspirate the wash buffer from the wells. Dilute the HRP-conjugated...

...the speed at which you need to spin your samples. For example, spinning down samples in 1.5 mL microfuge...using centrifugal force, which is generated by spinning the sample at a high speed. Being able to separate...that can accommodate different sized containers, spin at different speeds, or keep samples at specific... floor and can be about bench height. They often spin at similar speeds to their smaller cousins, but ... if applicable) listed in your protocol. Pro-Tip Spin speed is often given as Relative Centrifugal Force...and investigate the issue. When you are finished spinning your samples, carefully remove them from the centrifuge...

...NaCl PBS. Spin at 3000 rpm for 5 min at 4 °C and discard the flow through. Add your sample. Spin at 3500...0.1% Pluronic F68 PBS and incubate for 10 min at RT Spin at 3000 rpm for 5 min at 4 °C and discard the flow... Pluronic F68 PBS and incubate for 10 min at RT. Spin at 3000 rpm for 5 min at 4 °C and discard the flow...C, discard the flow through. Add more sample and spin 3500 rpm for 2–5 min at 4 °C, discard the flow ...that iodixanol is not easily removed. After each spin, add more formulation buffer and sample and make...

... NNGRR(N) Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant...

...pipette 2–20 µL single channel pipette Reagents Aspirating pipette, VWR 414004-265 5 mL pipette, VWR 89130...Sigma L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher... to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration...exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up to 4 mL of ...divide it into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap...

...protect individuals from potential accidents such as spills. BSL-2 is different because it includes labs that...to protect your body and clothing from potential spills. Gloves should fit well enough to be snug to your...your sleeves into your gloves to prevent potential spills down your sleeve. This may or may not be possible...worn to protect your legs and feet from potential spills. If wearing open-toed shoes, cloth coverings or...