We narrowed to 582 results for: SUB;

...freeze-thaw-freeze cycle before, or otherwise been subject to mishandling?  Is the experiment you’re planning...the same assay. This is one of the most complex subjects in antibody-based protocols - but thankfully this...

...target certain sites, or make only a subset of base substitutions.  The need for speed: base editing ...

...results of all four of those tests and not just the subset of those that return a particular result. Anything...review occurs before results are known. The author submits the research plan (similar to a preregistration...

...It should be no surprise then that a lot of our subject matter comes straight from you, whether you realize...second review from someone less familiar with the subject for clarity. Our content covers a lot of very ...

...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...

...promoters commonly used in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit ... releases heat shock factor 1 (HSF-1), which subsequently binds to heat shock elements in the promoter...

...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside... genes that allow for the use of a particular substrate in defined medium, without which the cells cannot...

...for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-ligase IV); together with some...Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we do not describe these...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...analysis of the kinetics of chromatin regulation and subsequent mathematical modeling. Its reversibility allows...

...caution, since the difference between studies is substantial (e.g, the poor performance of mRuby2 is detected...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...photo-reversible protein-protein interactions can control subcellular localization processes in vivo. They deposited...

...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Assembly of plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from...

...other references to scientific literature to substantiate the facts upon which it builds. This means you...need to be on a network with an institutional subscription), archived on PubMed Central, or in preprint...

... which proteins assemble into functional multi-subunit macromolecular complexes, but a serendipitous discovery...sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2 (1998):...

...experiencing sustained and substantial relief from both disorders. As of publication, submissions to the UK, EU, ...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...

...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...want to consider using an aliquot that has been subjected to a freeze/thaw cycle for titering as this will...