...nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization varies... change in response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al....study is the method’s sensitivity. The authors estimate that a minimum of 150-200 fluorescent protein ...phototoxicity concerns and extending the potential imaging time for this technique. The NIH Common Fund has made...epigenetics and cellular reactions to external stimuli. It could also have major applications to cancer...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Chen B, Gilbert LA, Cimini ...

...videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time. After taking...preconceived obstacles, optimistically grasp at limitless potential, and who sometimes wish they could live...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...practically universally loved (except for those rare times that we step on them with bare feet), to connect...

...during this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge...continue reading for more details. How much time do you spend reading scientific literature? Very...experimental results but, obviously, this is not optimal. What if you could save the figures of interest... instantly and are editable by the author at any time. Insights contained in your ReFigure could be beneficial... Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments Use video to share your...

...s Lab (plasmid page here). Now I spend much less time wrangling bacteria and instead help Addgene fulfill...Addgene? So why did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like...Outreach Scientist where I spend a large amount of my time meeting with and talking to graduate students, post-docs...teenage years I was super shy - there was even a time when I was too nervous to ask store clerks for help...graduate work at Harvard, I recognized that I would ultimately want to go into science communication but thought...writing, teaching, presenting, analytical thinking, time management, information distillation, etc) are transferable...

...more practical lab experience to later gain a full time position in a lab. I worked on the genetics of Wilms...of interest before you dive straight into a full-time position. For my part, I really enjoyed some aspects...the company was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on...has been rapidly growing. These factors left me optimistic that I would find a genetics related role soon...situation, communication between departments was sometimes difficult and the solution attempted was more ...solved with good management practices, but can sometimes go unnoticed at large organizations. Another downside...because you need to work independently most of the time and plan your own work schedule.  I really love ...

... other reagents. Virus distribution came up time and time again with many researchers lamenting the viral...Instead of spending time and money producing virus from select vectors in the repository yourself, you...frustrating process that requires a large investment of time and resources for labs that don’t have experience...titering, and testing viral preps for them. At the same time, we’re developing ourselves as a knowledge base ...to be distributing new types of materials. At the time of publication we’ve already fulfilled more than...

... Working in a lab is nothing like a typical part-time or 9-to-5 job. I had to ask to find out what a “...Professor, Assistant Professor, Associate Professor Sometimes teaches graduate and/or undergraduate classes ...that not all advice is good advice, and it is ultimately up to us to decide whether we want to take it...mentor and choosing the right lab. Remember that your time is valuable! Don’t jump on the first opportunity...whether it’s something you want to dedicate your time and energy to. How can I make the most of my experience...to ask “dumb questions” and to make mistakes. Ultimately, though, scientists are all just people. I’ve...cultivate stronger relationships with my labmates. Sometimes what I thought was a dumb and very basic question...

...individual MTAs every time they wanted to share materials, and the process was time-consuming and lengthy...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than...transfer office is sent a deposit agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes...when biological reagents are shared — both at what time and with whom. Tracking these connections allows...refined as new technology becomes available. Our one-time deposit agreement means we only have to work with...

... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The...of southern England. While the length may be intimidating, Ruthefurd’s writing will capture the attention...cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing and Communications...International Finance. A book club could lead to smart, sometimes contentious, discussions. I presented the first...

...just do it myself” or micromanage –you don’t have time for that and you won’t be as productive. For example... to, or what tasks you assign, the manager is ultimately responsible for outcomes. Be ready to support...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project...dumb” and that it is better to ask before wasting time. Focus on results. What does success look like...delegation allows you to make the best use of your time and skills. It also ensures that other people in...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This...

...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... offer support. “It is an exciting and emotional time!” George Church tries to recruit people who are ... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists to ...

...value I could bring to potential employers. Over time, I discovered that I not only enjoyed the technical...a cell culture experiment myself would take less time than the seemingly burdensome task of explaining...strengths, and learning styles. For example, during my time as a lab scientist, I worked with a diverse team...when managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced... experienced this firsthand when it took me some time to adapt to the communication style of a new, junior...solid foundation in the basics of management. Invest time in understanding key principles through books, workshops...

...tools. How many times have these plasmids been shared in total? Just over 49,000 times! Their long-lasting...adventures...From moving office locations three times, to opening our UK office in 2014, to starting our...such as our second most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier... use in human cell lines, the Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair... version is the 15th most popular plasmid of all time, but was found to have a point mutation in the puromycin...

..., these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity is crucial for...need in the scientific community for efficient real-time Cas9 activity assays. Recently scientists have created..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...ratio of RFP to GFP expressing cells provides a real-time read out of Cas9 activity.  By using the RFP:GFP...reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency. So if you are...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

...rigorous studies will give false signals from time to time. False positives in original studies and false... provided in most published papers. The precise timing, order, or every combination of materials and treatments...preregistration Preregistration is the process of creating a time-stamped research plan in a public repository before...control, and you think there are four important times to measure that growth rate, you report the results...occurs during the course of the study, say a fifth time has unpredictably high rates, should be reported...

...out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...are your regular next-door microbes which grow optimally in moderate temperatures (usually defined between...quantity can significantly reduce the operational time for a given process. These are extremely useful ...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters...a repressor/inducer will have higher lag time due to the time needed for transcription/translation. Many...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

...as many journals as possible at the same time. Take your time picking the appropriate journal and explain...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that... both the referees (as they get credit for their time and effort) and the scientific community, as the... you are sending it to. It’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable monomeric form as determined by ...resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate...isolated and characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I ...maturation in cells compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H...photostability compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet...working with edited “pools” of cells, eliminating the time-consuming step of single-cell isolation. Agudelo...

... their cargo can begin, which all takes time. Though the time delay until expression is highly dependent..., Marlon R., et al. "Development and optimization of a real-time quantitative PCR-based method for the...AAVs tend to be very neuro-permissive (Issa, 2023). Time to expression AAVs have to infect cells, hijack ...target tissue, two weeks is the standard ballpark estimation. Once expressed, viral cargo is usually quite...