We narrowed to 931 results for: TIM;

...individual MTAs every time they wanted to share materials, and the process was time-consuming and lengthy...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than...transfer office is sent a deposit agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes...when biological reagents are shared — both at what time and with whom. Tracking these connections allows...refined as new technology becomes available. Our one-time deposit agreement means we only have to work with...

... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The...of southern England. While the length may be intimidating, Ruthefurd’s writing will capture the attention...cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing and Communications...International Finance. A book club could lead to smart, sometimes contentious, discussions. I presented the first...

...just do it myself” or micromanage –you don’t have time for that and you won’t be as productive. For example... to, or what tasks you assign, the manager is ultimately responsible for outcomes. Be ready to support...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project...dumb” and that it is better to ask before wasting time. Focus on results. What does success look like...delegation allows you to make the best use of your time and skills. It also ensures that other people in...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This...

...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... offer support. “It is an exciting and emotional time!” George Church tries to recruit people who are ... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists to ...

...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable monomeric form as determined by ...resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate...isolated and characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I ...maturation in cells compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H...photostability compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet...working with edited “pools” of cells, eliminating the time-consuming step of single-cell isolation. Agudelo...

..., these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity is crucial for...need in the scientific community for efficient real-time Cas9 activity assays. Recently scientists have created..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...ratio of RFP to GFP expressing cells provides a real-time read out of Cas9 activity.  By using the RFP:GFP...reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency. So if you are...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

...rigorous studies will give false signals from time to time. False positives in original studies and false... provided in most published papers. The precise timing, order, or every combination of materials and treatments...preregistration Preregistration is the process of creating a time-stamped research plan in a public repository before...control, and you think there are four important times to measure that growth rate, you report the results...occurs during the course of the study, say a fifth time has unpredictably high rates, should be reported...

...out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...are your regular next-door microbes which grow optimally in moderate temperatures (usually defined between...quantity can significantly reduce the operational time for a given process. These are extremely useful ...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

...value I could bring to potential employers. Over time, I discovered that I not only enjoyed the technical...a cell culture experiment myself would take less time than the seemingly burdensome task of explaining...strengths, and learning styles. For example, during my time as a lab scientist, I worked with a diverse team...when managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced... experienced this firsthand when it took me some time to adapt to the communication style of a new, junior...solid foundation in the basics of management. Invest time in understanding key principles through books, workshops...

...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters...a repressor/inducer will have higher lag time due to the time needed for transcription/translation. Many...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

...tools. How many times have these plasmids been shared in total? Just over 49,000 times! Their long-lasting...adventures...From moving office locations three times, to opening our UK office in 2014, to starting our...such as our second most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier... use in human cell lines, the Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair... version is the 15th most popular plasmid of all time, but was found to have a point mutation in the puromycin...

...as many journals as possible at the same time. Take your time picking the appropriate journal and explain...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that... both the referees (as they get credit for their time and effort) and the scientific community, as the... you are sending it to. It’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

...the myriad challenges we encounter. As you spend time in a lab learning techniques and taking on independent...assessed potential protocol modifications, and ultimately decided on the changes to implement. As with ..., there will always be room to improve. The next time you face a problem, try to be mindful of how you...yourself to think of more creative solutions. Ultimately, problem solving is less about technical know-how...both technical and transferable (i.e. teamwork and time management), your ability to solve problems efficiently...Collaborating with others outside of your organization Time management Managing a team Leadership Resources...

... their cargo can begin, which all takes time. Though the time delay until expression is highly dependent..., Marlon R., et al. "Development and optimization of a real-time quantitative PCR-based method for the...AAVs tend to be very neuro-permissive (Issa, 2023). Time to expression AAVs have to infect cells, hijack ...target tissue, two weeks is the standard ballpark estimation. Once expressed, viral cargo is usually quite...

...tried-and-true tools means less time getting up and running and more time working on interesting biotechnology... a request for a particular plasmid and now it’s time for our lab technicians to retrieve the sample and...procedures. With this data in hand, we can go back and optimize our procedure to make the whole process work more...Barcoded materials make everyone's life easier, the time and capital cost of getting up to speed with a setup...Bioinformatic Analyses Use Software Apps to Save Time and Track Your Experiments Additional Resources...

...reading through an article for the first time. The first time through, just get an idea of the techniques...with the scientific literature a little bit at a time. Searching for literature My go-to search engines... the jargon and background in your field, but sometimes papers are also just poorly written. By nature...connection or flow among the figures and results that ultimately builds up to an overall conclusion(s). In some...literature in your field of interest. Set aside some time each day or week to at least scan the titles of ...

... are financially accessible. Sometimes only a few hours of coaching time can make an enormous difference...volunteer mentors are only able to go so far with their time and advice. A professional coach is paid to carry... can take some research.  It helps to spend some time thinking about what your goals are for the coaching...Coaching. One nice thing about a group is that sometimes this offers the option of matching with the best...a good match as a coach for many scientists.  Sometimes it can be helpful having a coach that can offer...

...have no idea how to write a grant!”, “I don’t have time for this if he wants data by next week!”, and “This...administrative side of the grant to be established. Take time to read and fully understand the RFA. The RFA contains...current state of the art technologies. Therefore, take time to review the literature and offer educated insight... will address the research challenge. Now is the time to make bold statements. While some of the statements...supplies, personnel, equipment, travel, etc. As a first time budget writer, you may find this section challenging...feedback on the application. Make sure to invest time and consider the reviewer's comments. Whether the...

...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression....directly for drug screening or easily shuttle the optimized, expression validated GPCR sequences to any desired...strains remains seriously limited, as prior optimizations are required for each individual target species...being generated, there is a need for genetic optimization of industrial yeast strains. However, industrial... the well-known yeast two-hybrid assay are not optimal for studying native mammalian protein interactions...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...

...: you only need to express two gRNAs at the same time. One system you could use is pX333 from the Ventura...follow these same assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing...amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors. The system is ...destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available for both monocot and...incredibly fast protocol, with only 5 hours incubation time needed for a single round of editing, and a subsequent... paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for efficient germline and somatic...Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...