We narrowed to 752 results for: UTY

... room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it...’ve enjoyed seeing scientists sharing their contributions on social media as well the excitement amongst...community. For example, we started a new service distributing ready-to-use viral preps in 2016 and we just...

..., is a repository for monoclonal antibodies, distributing over 3,000 antibodies and associated hybridoma...1986, to enable basic research by banking and distributing monoclonal antibodies at cost. We distribute...the University of Iowa. The minimal fees from distribution funds our operation. Antibodies are glycoproteins...

...directly collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...

...are more than welcome in contributing to The Addictive Brain. A contribution can either be an article,...Brain to spread awareness about how scientists utilize tax-payer money and government funding for research...

...optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a dark room with...microscope. Less fluorescence admittedly, but still so beautiful! Enabling the scientist in everyone - everywhere...costs, deep tissue penetration, and high spatial resolution are benefits of this technique. It is even possible...

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the...far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal fragment of Cas9 while...

... data. Here are some tips to help you in your execution:  Accuracy is not as important as precision for...need to run a validation assay in which serial dilutions for the target and housekeeping genes are run ...plotted against the log input concentration for each dilution. For the ΔΔCT method to be accurate, the absolute...Palais R.; Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of...

...more sustainable products Recycling is great for rerouting waste from the landfill, but what about all the...do. Remember, it was only 50 years ago that we routinely dumped toxic waste into the oceans, a practice...continue to examine our behavior and innovate solutions and practices to meet higher environmental standards...

... the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards of that..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...

...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...community and contribute in a way that everyone could utilize what we had made,” Baird said. “It won’t sit in...scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael...

...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence...ultrasound waves as opposed to light to produce higher resolution images in vivo. “One of the reasons we can’t ... less. As a result, ultrasound enables higher resolution images at depths up to eight millimeters – not...

...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...

...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

...information and, of course, plasmids. My primary duties are to manage this very blog (you’ll have to let...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting... about Management for Scienitsts from Addgene Executive Director Joanne Kamens ...

...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC....mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance...quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...

...on being financially self-sustaining through distributions of research materials, such as plasmids and ...new project to reach a sufficient number of distributions to cover costs, which can limit expansion into...” says Dr. Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the ...

...relevant kinetic analysis, but the trick is to utilize a second immobilization method. One possibility...efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds. We generated...folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely ...

... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering...