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Addgene

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We narrowed to 743 results for: UTY;

Showing: 261 - 280 of 743 results

  1. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...directly collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

  2. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...

  3. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a dark room with...microscope. Less fluorescence admittedly, but still so beautiful! Enabling the scientist in everyone - everywhere...costs, deep tissue penetration, and high spatial resolution are benefits of this technique. It is even possible...

  4. Science Communication for Everyone

    Type
    Blog Post
    ...are more than welcome in contributing to The Addictive Brain. A contribution can either be an article,...Brain to spread awareness about how scientists utilize tax-payer money and government funding for research...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    .../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the...far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal fragment of Cas9 while...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ... data. Here are some tips to help you in your execution:  Accuracy is not as important as precision for...need to run a validation assay in which serial dilutions for the target and housekeeping genes are run ...plotted against the log input concentration for each dilution. For the ΔΔCT method to be accurate, the absolute...Palais R.; Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of...

  7. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...more sustainable products Recycling is great for rerouting waste from the landfill, but what about all the...do. Remember, it was only 50 years ago that we routinely dumped toxic waste into the oceans, a practice...continue to examine our behavior and innovate solutions and practices to meet higher environmental standards...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    ... the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards of that..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...

  9. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...community and contribute in a way that everyone could utilize what we had made,” Baird said. “It won’t sit in...scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence...ultrasound waves as opposed to light to produce higher resolution images in vivo. “One of the reasons we can’t ... less. As a result, ultrasound enables higher resolution images at depths up to eight millimeters – not...

  11. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...

  12. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

  16. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...relevant kinetic analysis, but the trick is to utilize a second immobilization method. One possibility...efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds. We generated...folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely ...

  18. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering...

  19. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... plasmids: Twitch sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance...tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes, too, making...sensitivity. He and his colleagues came up with a solution: they devised self-associating fluorescent domains...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...not convinced that your project is an innovative solution with a strong impact, or a journalist does not...taking a break and incorporating EAs into your routine.  Getting started: Planning your career path and...informational interviews to gain insight into the daily routine of the potential job Join LinkedIn and other ...
Showing: 261 - 280 of 743 results