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We narrowed to 770 results for: VARS

Showing: 261 - 280 of 770 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... using a dCas9 variant as well. Furthermore, they provide a high-fidelity (HF) variant of the BE3 that...Streptomyces  Streptomyces bacteria produce a wide variety of bioactive natural products. To easily explore...of essential and non essential genes in a wide variety of bacteria including in pooled, genome-wide, formats...bacteria. The authors were able to engineer a dCas9 variant fused to bacterial enhancer binding protein required...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...Cas protein variants may also be useful for targeting ncRNAs. For example, the Cas12a variant can cleave...genomic elements (Hazan and Bester 2021). Other Cas variants Another option is CRISPR/Cas13, which targets ...specifically edit or degrade targeted lncRNAs. The Cas14 variant is a smaller and more versatile option. It can ...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...results. Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different... tend to have a strong signal and to work in a variety of applications. The desired specificity of your...your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies ...factor Biological systems tend to be complex with a variety of proteins interacting. Experiments tend to be...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...concentrations in different subcellular compartments can vary greatly. Additionally, the calcium changes you will...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...advantage: tissue-specific targeting. By employing various AAV serotypes, researchers can precisely direct...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...or RNA) to target cells has been challenging. A variety of vector delivery technologies have been investigated...vectors great gene delivery tools? There are a variety of key properties that distinguish AAVs as gene...multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms of AAV capsids...are under consideration, recent advancements in various AAV technologies have vastly improved AAVs’ potential...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...of yeast terminators of varying strength. A discussion of the effects of various terminators in the context...important role in RNA processing and contributes to variability in RNA half-life, and ultimately gene-expression...transcript. The purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally...

  8. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...proteins (FPs) came into wide use, there were a variety of ways to monitor cell, organelle, and protein...progressively longer blue fluorescence maturation times and varied blue-red maturation times (Table 1). These separate...useful for monitoring cellular events occurring at varied time scales.  Table 1: Monomeric FP Timers and ...and release from the plasma membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...determine the titer of your viral vector solution. A variety of titering methods have been developed to determine...viral particles that can transduce cells, which can vary based on target cells and can be altered by freeze...genome. While ddPCR is superior in precision and variability, qPCR is the more widely used method due to the...Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV ...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...such as Novartis, have specific web sites for postdoc listings, so the method of posting varies between...AstraZeneca, you are expected to publish, but this can vary depending on which company you choose. If publishing...postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, New England Biolabs, Regeneron...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains such as transcriptional...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...control which gRNA becomes complexed with which variant of Cas9. To circumvent this problem, previous studies...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...FPs just keeps getting larger with hundreds of variants available. Read on to familiarize yourself with...emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP...YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's...with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) ...

  13. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...everyone else?  In truth, people are incredibly varied in how they prefer to consume information. Personally...availability to resources (including the internet!) can vary depending on factors like socioeconomic status and...smartphones with low data requirements.  No visuals. Variety of hosting platforms can splinter audience. Primarily...areas of expertise.  Social Media Low to high Variety of options from text to video. Easy to target audiences...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...used for the production of Fab fragments where variable chains are secreted into the periplasmic space...Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018).  Recombinant...coefficient of IgG is around 1.35, though this can vary depending on the specific amino acid sequence (Maity..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies...2013). Mutations that affect this interaction to varying degrees determine the clinical severity of different...et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu,...

  16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...cell, instead of fluorescent markers. There are various types of SSC and FSC that can be used for this ...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...Jhunjhunwala, S., Aresta-Dasilva, S., Tang, K., Alvarez, D., Webber, M., Tang, B., Lavin, D., Veiseh, O...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...transduction efficiency. Transduction efficiency can vary with titer, cell type, and other factors, so make...libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled libraries...

  19. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ... different proteins and small molecules from a variety of sample types (Engvall 1971). Scientists value... the concentration of antigen in the sample. A variety of chromogenic, chemifluorescent, and chemiluminescent...direct ELISA is the quickest and simplest of all varieties. In this method, users immobilize the target antigen...antibody.  While the precise setup of the method can vary, typically users first coat the multiwell plate ...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ..., scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable...targets in one reaction, Cas enzymes, such as variations of Cas13 and Cas12a, are combined with different...CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche...Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12...
Showing: 261 - 280 of 770 results