We narrowed to 278 results for: alk

...ultimately set you up for failure. Don’t be afraid to walk away from a negotiation. If securing a faculty position...

... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

...declare your bench “RNase free” and refrain from talking to friends and colleagues while working with these...

...Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska Institutet, among ...

...R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence of Off-Target...

...choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks...

...that are essential for experiments but that nobody talks about. After months of troubleshooting, those people...

...dyes with different emission spectra to avoid crosstalk/crossover to reduce ‘muddying’ of the signal and...

... interest. Selected pathways  I attended a great talk when I was a PhD student. In particular, one slide...

...s best to consider these cases separately when talking about the tools that are available. Let’s consider...

...the intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the...

... and yes those purification columns we’ve been talking about!) are a significant source of waste in laboratories...

.... K., Roberts, N., Zhang, L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K...

... if you’re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is ...

...doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana KE. 1999. Quantitative RT-PCR: pitfalls...

...nucleoli, and little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins,...

...robust initiation of reprogramming (evaluated by an alkaline phosphatase-positive assay) as well as stabilization...

..., you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting...