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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 335 results for: c rel

Showing: 261 - 280 of 335 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this ...post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...covalent bond thus joining the two DNA strands and releasing topoisomerase (2).  These days commercially available...extra salt to your reaction: Topoisomerase I is released from the vector when the PCR product and vector...

  2. AAVED

    Type
    Collection
    ... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with Fellow Speakers and Attendees Friday...you will have final approval before anything is released publicly, but please only share materials that...

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial...genomes (Peters et al., 2017). Type I CAST systems rely on the Cascade complex minus its nuclease component... found to be particularly useful. But they were barely effective at editing eukaryotic cells — at least...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs... produce two biosensors that could quickly and reliably detect HMOs. Fatima’s first design is a whole ...enzyme breaks down the HMO of interest, the lactose released activates expression of GFP from the lac operon...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...arrays (e.g. for spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab...specific sites that allow them to be cleaved and released. These constructs tend to be smaller than constructs... If Csy4 is not expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the...recognize the tRNA sequences, cleave them, and release the gRNAs. PTGs are assembled into a wt Cas9-containing...editing of six different genes. This system doesn’t rely on any additional or host-dependent factors and ...

  6. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...depositing can be grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...) after three years on hold, Addgene staff may release the hold unless otherwise requested. Distribute...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

  7. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski...101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds to assemble...rational, straightforward design. Since the technique relies on base pairing, it has promise to be host-independent...

  9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters...heat shock. In the case of Hsp70, the heat shock releases heat shock factor 1 (HSF-1), which subsequently...cells and typically only in the same or closely related species from which they were derived. Similarly...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...long been extremely useful in the lab – they are relatively small and easy to work with, making them the ...10.1016/j.cell.2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun Y, Sun H, Zhang...the day, March 16, 2014, that I got my first preliminary results from a CRISPR genome editing experiment...

  13. Components of CRISPR/Cas9

    Type
    Blog Post
    ... tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9...use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) ...

  14. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    ...and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with...bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral infection, this nucleic...barring a loss of mitochondrial integrity that would release them into the cytosol. Fig. 2: Endogenous...upon viral infection. Endogenously produced dsRNA rarely reach this threshold and thus do not typically ...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

  17. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...python, R, perl, and ruby // This is a comment in C++ and Java Command line history: Tracking command...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

  18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...proven in (A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible...effort in preparation and practice is directly related to success presenting." A journey from graduate...LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business - Posted on Nature.com...

  20. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...functional contribution to a variety of behaviors related to addiction and pain. Once these are completed...neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration and...
Showing: 261 - 280 of 335 results