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We narrowed to 663 results for: epo

Showing: 261 - 280 of 663 results
  1. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...
  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...
  3. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...
  4. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...
  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...
  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification...
  7. Working 9-5: an Addgene Co-op

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    Blog Post
    ...scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ...
  8. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...
  9. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...
  10. An Addgene Summer Internship

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    Blog Post
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...
  11. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post
    ...stock. All of our proceeds go into running the repository and serving scientists better. That includes ...reagents Addgene makes it easy for scientists to deposit and request plasmids (via web interface, processes...
  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...
  13. Bacterial Expression Systems

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    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...
  14. Behind the Scenes of Addgene’s New Look

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    Blog Post
    ...so much more Addgene started out as a plasmid repository. Over the years, we recognized that what we offer...Addgene’s Blue Flame Award is prized among our depositors. Marking plasmids that have been requested over...
  15. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...Last month, the David Root and John Doench labs deposited new and improved libraries called Julianna, for...knockout screens. The Jonathan Weissman lab has deposited genome-wide CRISPR activation (CRISPRa) libraries...
  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...
Showing: 261 - 280 of 663 results