We narrowed to 304 results for: human dat

...cytoplasmic environments. However, approximately 40% of human (and most eukaryotic) proteins localize to chemically...suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5]. The moxFPs...of cysteine-free fluorescent proteins for the oxidative environment." PloS one 7.5 (2012): e37551. Pubmed...

...frequency to above 30% for a variety of targets in human cell lines, with an average indel frequency of only...catalyzes both cytosine and adenine base conversions in human cells. Nature Biotechnology, 38(7), 856–860. https...written by Mary Gearing in August 2016 and was updated by Jennifer Tsang in August 2020 and by Emily P... Browse CRISPR plasmids for base editing Find validated gRNAs ...

... of transcytosis and transduction using primary human brain endothelial cells. J Neurochem 140, 216–230...Aebischer, P. (2010). Efficient transduction of non-human primate motor neurons after intramuscular delivery...endocytosed material or molecules targeted for degradation. These two pathways also use different cytoskeletal...comprised of the viral proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been...

...between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette and sgRNA expression ...previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure 1: Validation of double... a simple method for selecting the guide RNA, validating its efficacy in vitro, and using it in mouse ... protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette reconstitution...vitro screening is a simple and fast method for validating your selected gRNA’s, and has the bonus benefit...benefit of supplying you with primers for validating your edit in mice once they have been generated (more...more on that later). Find Validated gRNAs to Target Mouse Genes To begin the in vitro screening process...

...synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like...usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether as part...

...other cancer mutations and non-genetic diversity in human cancer stem cells. Find the LARRYv2 Libraries here...Singh et al., 2025). Key to this method is their updated Lineage and RNA Recovery (LARRYv2) barcoding libraries...

... labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: ...instrument used in this reconstruction lay the foundation for all subsequent versions of LSFM microscopes...

...upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified...method allows for the recovery of a vast amount of data from a single experimental setup. As we’ve seen ...

... in medicine, 27% in natural sciences, 82% in humanities, and 32% in the social sciences go uncited. Creative...the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications ...example, learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about...at a time – A video by LeeAnne Thayer, a Ph.D. candidate in marine sciences at the University of Maine,...

...Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for...Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik January ...

...genetic screening. gRNAs targeting every gene in the human genome for example, can be made easily and cheaply...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...through the creation of innovative technologies. To date his group has develop several popular CRISPR-based...

...Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens junctions beta-Catenin...Lck-mTurquoise2 Plasma membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I...Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-...pLCK-mScarlet3_C1 Plasma membrane Lck N-terminal lipidation signal mScarlet3 Dorus Gadella 61180 pCMU-PMr...

...expression atlas of miRNAs and their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878....methods are often quicker and the repositories of validated small RNAs are vast and difficult to beat in terms...knockdown cells lines, and you can usually find validated shRNA sequences for your intended target. RNAi...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

... al. "Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7... in fusion proteins. Since GFP is such a well-validated, all-round good performing probe, it is the first...components. For a review see Lotze et al. (2016). To date, small probes that are entirely genetically encoded...

...He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison of different...how they can be used, and how they’ve been used to date . If you have any questions, please write to us ...time, Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows...

...W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci...approaches, and each of these gRNAs will need to be validated for high on-target binding and low off-target ...Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research...

...antibodies against all human extracellular proteins with community-based validation. They also have plans...additional validated reagents, and, with these reagents in hand you’ve got a good foundation to begin experimenting...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...share data through their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all...biological researchers deal with larger and larger datasets and use computer based analysis techniques, an...allow researchers to share code they’ve used for data analysis while other organizations like Dryad make... of causation from initial hypothesis through validation are easier to understand. The need for great ...

...applicable to industry if this path interests you  (e.g. human disease or models of disease, drug properties, etc.... Gather data on the lab choices How do you find out what the lab is really like? Gather data of course...me when the most analytic scientists seem to toss data-driven reasoning out the window when making decisions...for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni as you can or schedule...meetings can be the most informative way of gathering data. Former lab members can give open, revealing information...humiliation, accusations regarding lack of effort, intimidating use of discipline or competence procedures Threats..., persistent teasing, name calling, insults, intimidation, sexual harassment Isolation – including preventing...

...heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology Reports, 50(5). https...ubiquitin ligases in the degradation of CFTR, specifically UBE3C. Degradation of CFTR was measured through...components of the degradation pathway. HiBiT enabled the authors to observe degradation as well as endocytic...analyzing real-time kinetics, such as binding, degradation, trafficking (Hall et al., 2012). Antibody-based...combined with the HiBiT tag to provide complementary validation of protein expression and localization. HiBiT-tagged...ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins involved in endoplasmic...) used HiBiT to characterize the proteasomal degradation of cystic fibrosis transmembrane conductance ...

...screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https:...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR... of DNA. However, several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to...targeted delivery of Tet1 alone, or coupled with DNA oxidation and repair factors that allow for increased gene...Modification   Leah Schwiesow contributed to updating this article. References Haldeman JM, Conway AE...Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications...