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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 537 results for: invs

Showing: 261 - 280 of 537 results

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... using droplet digital PCR Droplet digital PCR involves partitioning a PCR reaction mixture into approximately... Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet rain in the...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ... in using the 3rd-generation system is that it involves transfecting four different plasmids into the ...quickly check that a construct is functional before investing time and resources into making a stable cell line...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vectors? The production of viral vectors typically involves shuttle vectors, also sometimes called transfer...target cells. However, while shuttle vectors are involved in the production of viral vectors, we consider...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...in a competitive ELISA the reporter signal is inversely proportional to the level of antigen in the sample...in a competitive ELISA the signal intensity is inversely proportional to the amount of antigen in the sample...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as ... we appreciate the opportunity to share these invaluable reagents with the scientific community. Note...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    ... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...technology The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistance testing is requested clinically, this involves growing a standardized amount of the bacteria ...focus on creating new antibiotic therapies, and investigate more effective drug targets (ones that are less...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    ...culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes or to knock down ...term for a number of different applications that involve different types of small RNA molecules, such as...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

  20. March for Science

    Type
    Blog Post
    ...march is meant to inspire scientists to be more involved in their communities (something already encouraged...mathematical constant “pi” supports scientists getting involved in politics. Disclaimer: The views presented ...
Showing: 261 - 280 of 537 results