We narrowed to 522 results for: pes;
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TypeBlog Post...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....
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Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...completely ineffective or results in low penetrant phenotypes that are hard to study. To overcome this, most...RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without the need... -
Antibodies 101: Conjugation
TypeBlog Post...multiplexing and can be used in a variety of assay types. Be sure to check the spectral properties of the... et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al.... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...antibodies are antibodies generated against antibody isotypes from a specific species, such as the rabbit IgG...primary antibodies are different species and/or isotypes. For highly multiplexed assays, using secondary... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...the neurons at specific firing frequencies. She hopes to identify how in vivo firing frequency of PV neurons...that this platform can be used to engineer many types of biosensors. This screening method allowed Tian... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...many studies, and could even be beneficial in some types of experiments (for instance, when the fluorescence...chemogenetics vs. optogenetics Find more about the many types of protein tags References on Addgene.org Check... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell an engineer... barcoded (this is pretty Addgene specific) The types or purposes of each given container, when applicable... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...amount of working memory resources. There are three types of cognitive load: intrinsic cognitive load is the...organizing and connecting information and a logic to the types and timing of questions that are asked. Many things... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose...drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes and metabolism... -
Plasmids 101: Cre-lox
TypeBlog Post...embryonic stem cells, and/or tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox... that are active only in certain cell or tissue types, during certain stages of development, or by making... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...addition, scientists have engineered entirely new types of plasmids without clear analogues in nature. For... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...studies in neuroscience where specific tissues, cell types, or even subcellular regions of a neuron need to...expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase systems... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...turns green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte...well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post... readers to more information if they desire it. Shapes. In this type of poster, written and graphical ...trickier to make figures fit nicely into circular shapes rather than a square or rectangular boxes. Background... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...then injected into a blastocyst. There are several types of genetically engineered mice: transgenic mice,...in part 2 of this blog series) controls the cell types in which the conditional recombination can occur... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...a paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following...fields of research they support. Monitoring these types of accounts can help you find papers related to ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves to promote...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...Prader–Willi syndrome, which have very different disease phenotypes, are two fascinating examples of epigenetics ... chromosome 15 are inactivated, but different phenotypes result depending upon whether the improperly ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...you to understand how to work with many different types of people both above and below you. During my time...of the NPA. You must accommodate many different types of people in order to harness their strengths and... -
Hot Plasmids: Summer 2025
TypeBlog Post...packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 offers broad tissue tropism for gene therapy...applications (Figure 4). Check out our blog post on AAV serotypes for more about the tissue tropism of these and...