Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,415 results for: ple

Showing: 261 - 280 of 1415 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...ligands to the same sample to label sub-populations of your protein of interest. For example, the Cohen lab...derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became...multi-component systems can be complicated to implement, they can be highly specific with a small and ...Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows...proteins in vitro, you can even use the same tag for multiple proteins but label each separately before reconstituting... assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled..., biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate synthetic...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...QCascade complex surveys the genome and binds its target DNA sequence. The transposase complex binds the...really depends on where a transposon lands. For example, in bacteria, transposons can move antibiotic resistance...is left behind. When the transposon-transposase complex finds its target site, it integrates, which produces...TEs You can think of autonomous transposons as “complete transposons” since they encode the protein they...selecting a transposon for a mutagenesis screen. For example, piggyBac and Tol2 are best for screening promoters...DNA-targeting module with the crRNA, 3) a transposase complex made up by three transposase proteins (TnsA, TnsB...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ...his program. Initially, Provost made his labs completely open-ended, allowing every student to work on... the culmination of many such experiences. For example, students in Provost’s course recently used a combination...Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased students’ confidence...Community (MCC) that seeks to reduce barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell...for your classroom, I highly recommend you try implementing one. It's likely only a matter of time before...Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...and replaced it with a double-bonded oxygen. This simple enzymatic reaction enabled conversion between the...and one-ring pyrimidine base. This is a far more complex chemical task, not a job for a single enzyme. ...bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence, and (3) a protospacer-adjacent...that site is filled depends on cellular factors. Multiple labs tried to identify beneficial factors to include...separate and preventing repair based on the complementary strand. This could explain why the edited U ...original G is not primarily converted back to a complementary C. Optimized base editing with eOPTI-CGBE and...so the Yidi Sun and Erwei Zuo labs tested and implemented several of these advances for CGBEs (Yuan et ...

  5. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...specific examples. Instead of saying “I have excellent communication skills” try giving an example, like ...application process, it's a good idea to talk to people in the field to understand what the norms are. ...match the job requirements, by giving specific examples of your work and explaining how your experience...pointing to your transferable skills. In the above example, if you don’t have experience with ddPCR, but do...beginning is often the hardest part, here are some examples to get you started! Dear Dr./Professor [last ...strengthened an application. Even so, there were examples of great hires who hadn’t submitted a cover letter... Multiple Authors...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...recombination intermediates can then resolve to complete the DNA repair process Homology directed repair...happen on the crossing strand (horizontally at the purple arrows) or on the non-crossing strand (vertically...is elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR... and even easy. This targeted-break technology coupled with HDR is a powerful site-specific knock-in tool... uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR was shown to increasing editing...series was designed to help explain the basic principles driving CRISPRs and genome editing technology...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand...

  7. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Optogenetics Ofer Yizhar New viral prep with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-GFAP-iGABASnFR2(no bind)...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-iGABASnFR2-WPRE AAV1...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-iGABASnFR2(no bind)-...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-flex-iGABASnFR2-WPRE...Biosensors GENIE Project New viral prep with multiple serotypes Looking for more viral vectors... Multiple Authors...

  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... forming the repeat:antirepeat duplex. tracrRNAs also contain multiple stem loops that are recognized ...Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an..., and the spacer sequence is used to target complementary DNA sequences. In laboratory settings, an sgRNA...whose sequence is not checked. Assuming full complementarity, its sequence matches the guide sequence. PAM...either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide and target...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...found the people at Addgene have become my friends, mentors, and some of my favorite people to talk to...student at Northeastern University, I am required to complete a six month work experience called a co-op to ...work of others. Having an environment filled with people who are interested in understanding the work you...Thankfully, my managers set aside time for me to complete an online social media certification on social...changes to our social media strategy. We ended up implementing some of these changes and seeing early results...results that suggest our content is reaching more people who need it!  After a few months, we wanted to share...

  10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR... bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR technology becomes increasingly...using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting this post...look at CRISPR’s potential for use in treating a complex genetic diseases, Alzhiemer’s disease (AD).  Troy...diseases with real phenotypic effect.” “It’s a very simple technology,” Doudna further explained. “That’s ...  Multiple Authors...

  11. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...the requesters what they needed. In the example above, for example, we fostered a formal scientific collaboration...uploaded to the preprint repository BioRxiv. This example clearly illustrates the power of collaboration,...choice but to quarantine in our houses, feeling helpless about the situation. But a few days later we had...certain projects. The idea behind the platform was simple: any person working on COVID-19 could file a request...groups. In other cases, the collaborations were simpler and the volunteers helped with things like translations...need help with your scientific research. It’s completely free, of course. You can find more information...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...while working remotely. Social distancing? Many people are now calling this “physical distancing” since... important to stay virtually connected with the people in your life. At Addgene, we’ve started virtual...SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing. Social...take-out) or volunteering to foster a pet, for example. If you are looking for some resources specific...activities that kids can do more independently. For example, they could do something like puzzling or crafts... time.  Find learning activities for kids. For example, Skype a Scientist is now open to families at home... future experiments and other researchers by implementing reproducibility best practices in your research...

  13. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...being one of the five senses? GPCRs or G-protein-coupled receptors (GPCRs)! Aside from these three senses...cascades.  So let’s break down the name "G-protein coupled receptor" into two parts: the receptor itself and...Takeda et al., 2002). G proteins: The receptor is coupled with heterotrimeric G proteins on the intracellular...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...finally, to a response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. ...

  14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... to determine precisely how the methods were implemented. We run into this problem frequently over the...not robust. Sampling noise There is, of course, simple noise in the process of sampling and inference-...problems above: unclear methods, QRPs, and small samples, then when replications do not come out the same...statistical tools that generate a p-value.  As an example, if you hypothesize that growth rates will be higher...answer those questions, the article is given “in-principle acceptance” to publish the final results regardless... special emphasis on positive controls and good sample sizes, concerns that are too easy to overlook when... worked with citizen scientists to design and implement authentic scientific research and as the Director...

  15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...techniques. These include reusing pipettes for multiple cell lines rather than using disposable ones or... of powdered media. Filtration does not ensure complete sterilization as mycoplasma can even escape 0.2... growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture, it...by providing proper training and guidance to the people working in the lab facility. This can be done by...mycoplasma infection from one cell line to another. Multiple cell lines should not be stored in a single incubator...cultures. The treatment can take a few weeks to a couple of months depending on the severity of the infection...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter... data gathering, regulatory efforts, policy implementation, program management, communications, diplomacy...setting: drafting legislation, making rules, and implementing the policy within the jurisdiction of the governing...working in science policy doesn’t have to be a complete career pivot. There are many ways to pursue your...your interests in policy or advocacy as a supplement to your career through engagement with your local...proposals. Check out the Day One Project for some example proposals and consider applying for their accelerator...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...disease estimated to affect 0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic... mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...process you would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas ... is most sensitive to your expected range. For example, calcium in the ER lumen reaches the sub-millimolar...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo. Neuroscience Research...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a channel, the ...expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nature Biotechnology... Multiple Authors...

  20. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four... more than one theme and can be used to answer multiple types of questions. Writing a rough outline of... say You’re going to be interviewed by numerous people during your industry interview. Each interview ...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...interviewer and then thank them once the interview is complete. Remember, your interviewers took time out of ...remotely Remote interviews are likely new to most people - even hiring managers and your interviewers. You...a question. Maybe you ask for questions after completing each section of your talk. Some video conferencing...
Showing: 261 - 280 of 1415 results