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Showing: 261 - 280 of 618 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena such as...

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...intensity. If your samples and controls were processed the same way, the GFP- population should overlap...samples and control underwent different treatment processes, you may see a shift in the negative population...

  3. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...quickly.  Through the years, we have perfected processes to curate the data our depositors share and enrich...and handle millions of queries. In an iterative process driven by our user experience experts, our software...

  7. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...analyze plasmids, which can help you visualize this process, or visit our restriction analysis blog post. ...now use next-generation sequencing during our QC process. This way, we can confirm the sequence of the entire...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...protein expression. Methylases involved with these processes (for example Dam and Dcm methylases) are independent...used for site directed mutagenesis. During this process, incorporation of a desired mutation into your ...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...understand, and the complicated and unique metabolic processes of plants are being investigated on limited funding... developmental, metabolic, and photosynthetic processes. Often the tools developed for plant molecular...

  11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... see how those mutations influence the disease process. In the Cell Stem Cell paper, for example, we generated... increase the efficiency of the genome editing process. The first really popular tool was the zinc finger...

  12. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    ...meant facilitating the Material Transfer Agreement process, which was a major barrier for depositors and requestors... requestors alike. We transformed this process by aligning institutions with standardized agreements and...

  14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment...
Showing: 261 - 280 of 618 results