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We narrowed to 535 results for: val

Showing: 261 - 280 of 535 results

  2. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...internal control (ATCC#VR-1516) until the assay is validated. Infectious titer: (Plaque Forming Units (PFU...Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity of adenovirus...

  4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Additionally, controls available in the system are validated and were chosen because of their broad applicability...process down into four easy steps, provides the real value for the user. Let's walk through designing a CRISPR...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibody options for your experiment (all well validated for your application, of course!) When the stars...these. After all, there’s no sense in spending valuable research dollars on a new secondary antibody if...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... off-target binding. The activity of SpRY was validated in double stranded cleavage and base editing applications... Flexibility History of CRISPR Cas – A Tale of Survival and Evolution  ...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ...details. Not only will these mStayGold variants be valuable tools for your next experiments, they will also...expression than AAV9. This new capsid will be a valuable tool for investigating NHP brains. Figure...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...common features of viral vector backbones; once validated these universal primers can be used to titer any...include defective particles. Studies have found that values can be 10-1000 fold higher than for functional ...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...contaminant is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544...purposely mixed another AAV vector with AAV-68544 to validate our method. We then extracted the DNA and processed...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...

  15. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting...Additional Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...

  16. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...Each hit from the screen should be independently validated to ensure that the desired modification produces...place. With the proper experimental design and validation practices, CRISPR libraries can help you learn...

  20. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... doesn’t have a Math & Science Night (or some equivalent), here are some quick tips to get things going...feedback on my presentation, she said “it was such a valuable extension to our curriculum and offered engaging...
Showing: 261 - 280 of 535 results