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We narrowed to 545 results for: val

Showing: 261 - 280 of 545 results
  1. Summer SciComm: Preprints

    Type
    Blog Post
    ...advocates have argued that while peer review is valuable, modern scientific publishing has a number of ...Burgess SJ, Schwessinger B, Hensel Z (2019) On the value of preprints: An early career researcher perspective...
  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...
  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...and going frequently - an environment ripe for valuable work and materials to slip through the cracks....working on interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, ...
  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...internal control (ATCC#VR-1516) until the assay is validated. Infectious titer: (Plaque Forming Units (PFU...Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity of adenovirus...
  5. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ... off-target binding. The activity of SpRY was validated in double stranded cleavage and base editing applications... Flexibility History of CRISPR Cas – A Tale of Survival and Evolution  ...
  6. Hot Plasmids February 2024

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    Blog Post
    ...details. Not only will these mStayGold variants be valuable tools for your next experiments, they will also...expression than AAV9. This new capsid will be a valuable tool for investigating NHP brains. Figure...
  7. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...antibody options for your experiment (all well validated for your application, of course!) When the stars...these. After all, there’s no sense in spending valuable research dollars on a new secondary antibody if...
  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ... can result in the retention of introns or the removal of exons. Disrupting promoter or transcription ...) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in...
  9. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ...Additionally, controls available in the system are validated and were chosen because of their broad applicability...process down into four easy steps, provides the real value for the user. Let's walk through designing a CRISPR...
  10. Tips for Titering Your Lentiviral Preps

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    Blog Post
    ...common features of viral vector backbones; once validated these universal primers can be used to titer any...include defective particles. Studies have found that values can be 10-1000 fold higher than for functional ...
  11. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent...
  12. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ...contaminant is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544...purposely mixed another AAV vector with AAV-68544 to validate our method. We then extracted the DNA and processed...
  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...
  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...
  15. Hot Plasmids: May 2026

    Type
    Blog Post
    ...protocol for expression and purification along with validation and expected results (Figure 1). Nearly 100 labs...specificity to be linked to gene expression data. In validating their method, they applied TCRAFT both to an ...
Showing: 261 - 280 of 545 results