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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 580 results for: vit;

Showing: 261 - 280 of 580 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... the known pathway that were essential for the activity of different ligands. Such knowledge could be ...effects. Typically the phenotype would be studied in vitro, but there is enormous potential in performing in...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...exposed ssDNA bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created... edits at certain positions within the editing activity window (Kurt et al., 2021). They hypothesized ...suggest that DSBs are a common intermediate of CGBE activity, and they are sometimes processed by error-prone...

  3. Validated gRNA Sequences

    Type
    Collection
    ...sequence and your genomic target may reduce the gRNA activity. Know your target. Which species or variant of...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ... a weak signal. However, they have high cross-reactivity, vary significantly from lot-to-lot, and have...but they have higher specificity, lower cross-reactivity, and are more consistent lot-to-lot. The highest...usually preferred because of their higher specific activity, slightly lower cost, and smaller size. However...

  7. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...

  8. Brain Initiative Collection

    Type
    Collection
    ... about our antibodies ID Recombinant Antibody Reactivity Recommended Applications PI Additional Resources...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...ways to incorporate science themes into our fun activities and Halloween is no exception. For the past 10...grasshoppers, including those linked to neurotransmitter activity and geotactic behavior (Biron et al., 2005).   ...

  10. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...microscopy, the team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain...hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these systems further...

  11. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...feels both welcomed and resourced for success. I invite you to join me here, and on my LinkedIn page, as...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

  12. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...neuroscience by granting targeted control over neuronal activity. While they serve similar purposes, both techniques...or track the exact time of activation. Receptor activity is also drawn out as drugs can remain present ...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ... really, the possibilities are endless.    Activity Example audience  Talking about your career...defining your audience for science communication activities  Many people are concerned that by defining a...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    ...clever system for reducing the on-target off-tumor activity of CAR–T cells was recently devised by the Yingxiao... plasmids here!   Wu Y, et. al. Control of the activity of CAR-T cells within tumours via focused ultrasound...

  20. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ... continue to be useful for monitoring neuronal activity in the form of calcium dynamics. (For more details...fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled neurons...
Showing: 261 - 280 of 580 results