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Showing: 141 - 150 of 246 results

  1. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2  Liu X, Zhang...by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen...chromatin by biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional ...

  2. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, ...Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein...its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O....cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org...

  4. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L.,...Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093...Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...

  7. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, ...blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J....blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Tet-On and Tet-Off systems: second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...
Showing: 141 - 150 of 246 results