We narrowed to 582 results for: Cit

...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog...

...the development and metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy... promote microenvironment’s malignancy but also incite different organs to become malignant. Exosomes ...

...big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...

...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...

...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

...though that’s what I thought,” she said. “I’m so excited to see where my life will take me. I’m also just...t had these conversations. Now, I am much more excited about where my career path will take me and much...

...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...

...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...

...in-depth In other tools, guides are ranked by specificity of targeting or by cut-to-insert distance for...determination (CFD score) is used to measure specificity of a guide to the target. The CFD score ranges...

...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...

...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...

...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...

... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...

...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...lot. The problem in plant science is there is a paucity of resources. We wanted to expand the toolkit. ...

...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...

...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...

...efficiencies of 90-100% (Fig. 2B), and >99% on-target specificity. Additionally, by expressing multiple crRNAs ...the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR toolbox for...