We narrowed to 399 results for: MUT
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TypeBlog Post...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...
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AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner... -
Lentiviral Vector Uses and Overview
TypeBlog Post...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due... -
CRISPR Antimicrobials
TypeBlog Post...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization... -
Plasmids 101: Protein tags
TypeBlog Post...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...contains the following mutations: Y444F, Y500F, and Y730F. AAV2(4pMut)dHS The AAV2(4pMut)dHS serotype displays...21):6229-39. PMID: 26310623 AAV2(4pMut)dHS When using the AAV2(4pMut)dHS serotype in future publications...spread following injection in the brain. The AAV2(4pMut)dHS serotype was developed by Shannon Boye’s lab...It is derived from AAV2 and has the following mutations: Y444F, Y500F, Y730F, T491V, R487G, R585S, and...from the AAV6 capsid and contains the following mutations: Y705F, Y731F and T492V. AAV44.9 The AAV44.9 serotype...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3)...citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ... -
Viral Production at Addgene
TypeBlog Post...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then... -
Transferable Skills Guide: Creativity
TypeBlog Post...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org... -
Hot Plasmids - August 2020
TypeBlog Post...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation... -
Lab to Office Culture Shock
TypeBlog Post...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers... -
Plasmids 101: Codon usage bias
TypeBlog Post...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...leverage another organization’s expertise to our mutual benefit. Our partnership with SnapGene, for example... -
CRISPR Activation: A Practical Guide
TypeBlog Post...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...