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We narrowed to 922 results for: Por

Showing: 421 - 450 of 922 results

  1. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...this technique, researchers have begun to make important discoveries in various biological fields. In 2015...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...

  2. An Introduction to Adenovirus

    Type
    Blog Post
    ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to...template for repair, which will result in the incorporation of your sequence, a.k.a a knock-in! So how does...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...molecule is used as the template, you need to incorporate homologous sequence to the right and/or left ...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ...analyzable transgenic in hand, so it is pretty important to get this process right the first time. I’ll...to transform when they begin flowering. It is important to perform the transformation not long after flowering...cut off the primary inflorescence (the flowering portion of the plant) near the base of the stem. This buys...s time to select for transformants. It’s very important to select multiple T1 plants from each transformation...

  5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and then used with ...attach a fluorophore at the specific site of incorporation (Figure 1d). Fluorogen activating proteins...excess dye from the sample, which is particularly important for samples where washing is difficult, such as...inside living cells. We recently discovered that incorporation of four-membered azetidine rings could substantially...

  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...interact with each other, which is critically important, especially for young researchers. It was a really...technologies. Majority of them are eager to just "import" technologies developed abroad, so it's a little...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... envelope allows the lentiviral vector to be transported retrograde and also has higher neuronal transduction...rabies glycoprotein. The glycoprotein will be incorporated into the replicating rabies virus envelope, ... lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral...0042 Takeuchi Y, Liong SH, Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Geneva (Creed et al., 2015) used optogenetics to support these existing findings by controlling neurons ... animal is placed into a tank of water with no support from below or anything to hold on to. In general..., 2007). A study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  9. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...number of systems that can be studied by providing support "for development of tools, approaches, and infrastructure...great for studying parenting strategies and make important ecological indicators since species live in water...Aiptasia - algae Breviolum minutum symbionts are important models for solutions to the coral reef crisis.... interesting biologically. They have economic importance as harmful weeds. The parasitic fusion is such...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    ... editing efficiencies in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and...., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on... more. For added precision, the authors also incorporated LOV-Turbo into TransitID experiments.  Find ...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...synthesis polymerase known to preferentially incorporate A bases across from abasic sites. This approach...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...a template — in this case, encouraging it to incorporate a new base across from the abasic site before...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...2024). They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and ...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus, Neisseria spp., Paracoccous...Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

  14. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...happening at Addgene under the hood. A significant portion of our development may not be visible to users ...but you have definitely reaped the benefits. We support everyone that works with Addgene in some way. For...the development process at Addgene. Diversity supports our innovation Our software development team is...are reusable software components that can be incorporated into software. You can think of these libraries...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...research. Open access journals also provide many opportunities. But these continue to rely on printed journal...years. Which leads to the question of how we can support researchers as they try to keep up with the growing...total view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts...the video abstract genre offers a low-barrier opportunity for researchers to leverage the video medium ...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... in advance Before validating your edit, it’s important to know a few things about your target cells and...goal is to generate a clonal cell line, so it’s important to verify that you won’t need to screen hundreds...in silico prediction analyses like CRISPRitz or CRISPOR can help narrow down the list of sites where off... integration into the guide RNA selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186...

  17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... targeting astroglia. It has additionally been reported to have tropism for lung, liver, vascular endothelial...engineered to infect neurons and undergo retrograde transport, traveling up the axon to the cell body and driving...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...long-read sequencing techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ...long-read sequencing (1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer... plasmid multimerization troubleshooting tips. Portions of this figure were made with BioRender. ...AE. Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go until completion. The time ...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...

  20. Antibodies 101: Validation

    Type
    Blog Post
    ...Reproducibility First, let’s review why validation is so important. Antibodies are a critical component of the researcher...or plan your own validation experiments, it’s important to know what you are aiming to achieve. In general...as whole cell lysate or tissue sections. It is important to confirm that your antibody is not binding to...proteins bound to the target protein, so it’s important to understand if protein:protein interactions ...

  21. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...or electrical (electric current forming membrane pores) transfection. These techniques are still used and...has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and the ...you clone your gene of interest into and is an important feature for your experiment; it must be chosen...using them.   Regarding biosafety, it is also important to be aware that lentiviral particles are a powerful...

  22. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to be labeled... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

  23. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... the University of Cambridge. One of the most important steps in the CRISPR experimental process is validating...silico prediction. These techniques differ in important ways, but can also complement one another by providing... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

  24. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... methods allowed immunologists to develop a spatiotemporal view of an immune response, but the study of...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...than the standard Ti:Sapphire laser. It is also important to finely tune the excitation wavelength as it...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent...

  25. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...Desktop Genetics .For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG ...into cells. Optimization of delivery methods (electroporation, lipofection etc) is the most crucial aspect...includes selection of transfection buffer and electroporation pulse program to achieve high transfection ...many factors. Some of these include: Most importantly, the crRNA should cut near the insertion site...

  26. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre...Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford)...

  27. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...public support for research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science...Outreach Scientist Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation...

  28. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... Find plasmids from this paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for ...

  29. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...popular videos. Describes fossil rocks and their importance in understanding biological history. 4. Please...   5. Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education...

  30. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Realizing that Addgene would be successful and the importance of lentiviral vectors in ensuring that success...international plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials ... Addgene's Future, the research team, and the importance of sharing information/knowledge Outro Many ...
Showing: 421 - 450 of 922 results