...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...salaries - in some cases reversing these decisions entirely. At Future of Research, we had been collecting...are going ahead with raises anyway, including the entire faculty of the biochemistry department at the University...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

... some 400 molecules that have been shown to be required by one or more NRs for efficient and timely activation...Primary or core coregulators (those that interact directly with NRs) exist in steady-state complexes with...the new version of the NURSA website will feature direct links to listings of available plasmids for our...relationship with Addgene for the benefit of the entire research community. Thank you to our guest bloggers...

...reporters specially designed for the aims and requirements of in vivo studies. A particularly useful sensor...or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7...various AAV serotypes, researchers can precisely direct the calcium sensor to specific tissues or even ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

...formation via NHEJ, precise modification with Homology Directed Repair (HDR), CRISPR activation or repression,... edits compared to DNA transfection.  Homology directed repair (HDR) with Nanoblades CRISPR-based HDR ...To utilize HDR, a donor template containing the desired sequence flank by homology arms must be delivered...with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm or...

...that the artifactual PSCs commonly observed with direct stimulation are largely absent in the soCoChR-expressing...BBa_J23100) and expresses N-demethylases and other required enzymes, including a glutathione S-transferase... ΔguaB E. coli are missing an essential enzyme required for conversion of xanthine into guanine, and without...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...

...engineering for each loci, CRISPR/Cas systems only require changing the programmable guide RNA. Gene activation... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...

...heard from friends that Scientist positions often required a one-hour presentation about a prior research...manager or recruiter what presentation format they require. Frequently they will ask you to present about ...similar answer in a following interview. Expect to be tired once the interview is complete Industry interviews... interviewers might.  You’ll probably still be required to give a job talk for a remote interview. Fielding...

...be dull and painful to sit through (imagine the entire audience playing Candy Crush or reading email).... interviews. The idea is to ask questions in a directed way to bring out these interesting stories and... happens, but get things back on track if the direction of the interaction is not interesting to the audience...politely but firmly get them to stop talking and redirect the conversation. The person you cut off might...

... a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing... or just random mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’...Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature Communications...

...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...determined experimentally. For larger libraries that require hundreds of nanograms of DNA, you will likely need...bacteria will be its own colony, and will not be in direct competition with other colonies containing smaller...

...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...is right for you outside of just the program requirements: by talking to current students about the work...

... steel wire into a backbone structure of a protein, based on its atomic coordinates. These wire models...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for BioMolecular Modeling and...

...students published their work identifying new genes required for stem and progenitor cell maintenance in planarians...Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their work and become...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance in planarians...

...description. You check off all the boxes - you have the desired degree, you have mastered the applications they...person. Similar reasoning may also apply to other acquired skills.” It is nice when other people point out... of a time you’ve lead your team in a defined direction in the past. Maybe there was a time you noticed...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

...screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but this tends to limit... (figure 4f) of the tTA inducer virus, but the desired sparsity of labeling is achieved with lose doses...-color labeling of neurons, because it doesn’t require the generation of a transgenic mouse. 2. Cell-... gene vectors for neurons, no transgenic mouse required (see figure 5 and supplemental figure 8 in Chan...

...also less accessible due to their expense and requirement for lab equipment. Plus they create plastic waste... discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits for different purposes...nucleic acids are released by dipping the cellulose directly into a PCR buffer, where DNA or RNA can be amplified...This method takes less than 30 minutes and only requires incubating the columns with 1M phosphoric acid...

...content-aware suggestion dropdown list will let you jump directly to the type of information you are looking for...straightforward search is now even easier with a direct link to the plasmid page at the bottom of the dropdown... dropdown list: The search bar will suggest direct links to other types of searches including depositing...search for “Melina Fan” and you’ll be able to go directly to a page to see the plasmids deposited by our...

...binding-dependent expression of the HIS3 gene, which is required for histidine manufacture and therefore growth...of interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset...at the same time in order to try and access the entire interactome of a set of proteins at once.  However...gain access to the nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin...

... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the familial...it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops prior... this saying is especially true. If your event requires a lot of set up or cleanup, ask for volunteers...

...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...ribosome. One resistance gene on your vector might require two different selection molecules! It all depends...option is to use a different method of selection entirely. Auxotrophic selection relies on the host organism...organism being unable to synthesize a nutrient required for growth. Before delivery of the vector, the deficient...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...single model. The first approach can lead to new directions, while the second approach could terminate with...think realistically about the time and resources required to feasibly accomplish the aims and clarify what... and Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia...

...Rett syndrome experience regression of previously acquired skills such as speech, coordination, purposeful...programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies...adeno-associated viral (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three ...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...

... the ideal codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization...necessary for proper protein folding (Stadler and Fire, 2011). Therefore, codon optimization may disrupt...1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential...doi.org/10.1016/s0165-0270(02)00362-x Stadler M, Fire A (2011) Wobble base-pairing slows in vivo translation...

...DNA strain which results in two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can...populations. To develop this method the lab was inspired by work that used an integrated GFP reporter that...fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE ...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...

...hiPSCs usually require about 5-10 passages. Furthermore, working with Sendai virus requires stringent biosafety...to the process of collecting fibroblasts, which require a more invasive skin biopsy procedure. This becomes...PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming by transformation-deficient Myc. ...

...skill developed during PhD training. Creativity is required to develop new projects and test novel approaches... all the funding in the world? What if you could hire more people? What would you do with unlimited time...over half of which, when implemented, impacted the entire company. It is important to note that the person...product team is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism...

...  Antisense RNAs encoded on the plasmid either indirectly inhibit replication through inhibiting translation...translation of replication machinery proteins, or directly inhibit replication by binding to the origin of...sequences within the origin of replication and are required for replication in plasmids that contain them ...Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic...

... and therefore may need fewer resources for the entire translation process. For example, during high growth...inappropriate locations or fail to make it through the entire transcript resulting in the production of nonfunctional...that their codons are more appropriate for the desired expression host. This is known as “codon optimization...Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...

...Ciuffi, 2012). One family of viruses, retroviridae, require integration for their life cycle to continue. They...IN (integrase). These viral packaging genes are required for the production of lentiviral particles.  The... keep in mind is that titering these IDLVs may require a slightly different protocol than regular lentiviral... (2008). Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following...

...variety of behaviors, opening up new research directions that were inaccessible with earlier tools. They... in localization to the cell membrane and not a direct change in the fluorescent properties, pYtags are...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...temperature indicator B-gTEMP designed to meet those requirements (Lu et al. 2022). By fusing two bright fluorescent...