We narrowed to 742 results for: SON;

...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...

...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...

...protocol to remove endonucleases activity. For these reasons choosing the correct solution is fundamental, and...

...restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately chosen ...

...Ranjith-Kumar, C. T., Guchhait, P., & Surjit, M. (2025). Comparison of immunogenicity and protection efficacy of ...

...easy to spot and even easier to ship back. Be that person! If you use serological pipettes from Corning...

...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

...This series was written for selfish reasons: I wanted to learn about careers in science communication...

...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...

...the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals...

...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

...tips for applying it in your own lab. Table 1: Comparison Between Standard Restriction Cloning and Fast...

...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

... Lianna Swanson...

...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...

...she will start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility...

... Rachel Leeson...

...stop codons.    New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...

... light.   Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ...

...mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity...

...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...

...strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-...

... color palette was chosen to help convey our personality — Addgenies aren’t afraid to have a little fun...

...Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene...

...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression...

... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal...

...the gut-brain axis (admittedly for self-serving reasons – I want to understand my obsession with the combination...

... Rachel Leeson...

...they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you...

...     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,...

... Lianna Swanson...

...shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins...

...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and...

...functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward...

...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...