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  2. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem Cytochem...

  3. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...involved “plates,” and our scientists and lab technicians always knew from context what a “plate” was. ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...error rate of current NGS approaches allows for a detection limit of roughly 0.1%. Thus, rare, pre-existing...prior to treatment, there are small (previously undetectable) populations of cancer cells that are resistant...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane...

  10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...10.1021/acssynbio.9b00395 O’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935...as time-lapse imaging or with high light power detection of molecules.  Lee, J. et al., Science Advances...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    ...network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...different labs during your career; you learn new techniques and interact with new people. If you are looking...

  12. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

  13. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

  14. Magnetic Control of Proteins: More than a Dream

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    Blog Post
    ...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was...

  16. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...your plasmid prep We'll discuss the following techniques to increase plasmid yield. Keep in mind that ...that you might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

  21. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...

  22. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...best teachers and mentors pass on more than just technical knowledge, shaping their trainees’ very way of.... The MCC develops and makes freely available technical and pedagogical resources, offers mentoring for...

  24. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post
    ...them that their findings may be the result of a technical artifact that introduced an ancestry bias into... a reduction in the number of the dependencies detected. Given this new information, Misek notes that ...

  26. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Sanger sequencing-based analysis tools are unable to detect or analyze complex edits, such as those generated...sample, ICE includes visual representations of all detected edit types in the sample. The visualization helps...

  27. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...

  29. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...

  30. Lab to Office Culture Shock

    Type
    Blog Post
    ...transferable skills. Transferable skills are the non-technical skills you pick up wherever you work, and you’... communicating. After years of focusing on my technical skills with cloning and lentivirus production,...

  31. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...the presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid...Danino, Tal, et al. "Programmable probiotics for detection of cancer in urine." Science translational medicine...

  32. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  33. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  34. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...Escherichia coli and Agrobacterium tumefaciens. 3 Biotech, 13(3), 91. https://doi.org/10.1007/s13205-023-...

  35. Bioinformatics at Addgene

    Type
    Blog Post
    ...Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository continues to expand, we realized... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  37. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...addition to certain staff scientists and those in technical roles. Therefore, institutions are responsible... law. However postdocs, staff scientists and technicians in primarily bench-science positions are not....

  38. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...
Showing: 561 - 600 of 797 results