...million dollar sales pitch. Given this large sum, one might expect that applicants would work for months to ...research? This is the central idea. It should be at a high level, broadly understandable to scientists outside...engaged by your presentation, then you’re on the right track. Furthermore, practice getting back to your...

...data inside the ELN is reproducible, and only the highly restricted number of people who can access the ...practical to move all this data in bulk into a lightweight tool like an ELN. It is nevertheless critically...

...particular gene. Furthermore, a target sequence may have high homology elsewhere in the genome. These off-target...Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol 19...Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature 556:57–63 . https://doi....

...depositing lab. (For our more popular libraries, you might receive a similarly-sized aliquot from an amplification...ll want to infect your cells with the virus–the right amount of virus, that is. Ideally, each cell will...Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9...

...recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through...Other investigators who had been working to develop high efficiency gene targeting approaches improved on...repair, it has proven more challenging to develop high efficiency CRISPR-based approaches for the generation...

.... This over-reliance on the perfect story for a “high impact” publication can lead to the publication ...reproducibility issues in the biological sciences. I’d highly recommend listening to the panel discussion to ...Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists on...

...important that your gRNA sequence is designed to be highly active and reduce off targets. For CRISPRa and ...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires...genetic screens using genome-wide libraries have shown high concordance between different sequences targeting...

... LITE system, which uses light to regulate transcription, also includes light-regulated histone methyltransferases... CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements...

...may be a good choice for targeting neurons, with high transduction frequencies in the central nervous ... our in-stock viral prep service.  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid...AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency, and is an excellent choice...

...using them. Indeed, the future of mouse work is bright as it is now easier than ever to manipulate the...technology, CRISPR, that is simple in concept, straightforward to use, and robust in performance. In its natural...BL, Xavier RJ, Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation...

.... PubMed PMID: 18845712. 9. Warren, L., et al., Highly efficient reprogramming to pluripotency and directed...PubMed PMID: 26189426. 15. Si-Tayeb, K., et al., Highly efficient generation of human hepatocyte-like cells...Central PMCID: PMC2946078. 16. Zhang, D., et al., Highly efficient differentiation of human ES cells and...

...existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section...

... an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...

...protein for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment  Hot Plasmid...Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the pPSU Plasmids...

...generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various...

...resources! Ready to sing along with this year's lineup? Right! We're ready to, so let's get started!  On the...sequences🎵On the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation...

...adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes... bit of everything: frog snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters...

...from the first generation of base editors to the eighth generation (and dual base editors, too!).  On the...gave to me: CRISPR methods for bacteria.  On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR ...

... In each short episode of this series, we’ll highlight one tip that we’ve learned in the lab that will...

...to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A... The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598...

... world making breakthrough discoveries!” I knew right then I had found my dream job, because one thing...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...

...rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create a plasmid...rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made...

...every new lab mate? Do you have a trick to get the highest yield out of your minipreps? If so, we’d love to...Addgene Blog Possible guest topics Some topics you might consider are: An overview of software tools useful...

...each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In the future, you’ll be...Domain Constructs Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera...

...account, simply click on your account name in the top right corner of PubMed to navigate to your account settings...on the “Filters & Icons” link (see image to the right). Once on the “Filters” page, select the category...

...science-themed submissions: string lights hung up in the shape of RNA, light-up neurons on the lab door, and...

...aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested... repository (you can find the link in the upper right hand corner of the dashboard). Therefore, users ...

... 2016, with the goal of providing easy access to high quality, commonly used viral vectors. Of course,...course, many scientists also need easy access to high quality, not so commonly used viral vectors. That’s where...

...Data Hub If you're trying to decide which AAV is right for your experiment, searching the Data Hub's curated...experiments, let us know ([email protected]) and we might just send you a new Addgene Data Hub t-shirt!    ...

... we mention graphs such as the one shown to the right that illustrate a grad student's happiness v time...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

...hear Dr. Koeris explain however, the time was not right for the development of phage-based therapeutics....developing products that are needed but that are high risk? 34:37-36:53 : Was It difficult for Dr. Koeris...

...that is and has been to publish in journals with a high “impact factor,” a measure that dates back to 1975...Non-Elite Journals” found that the proportion of highly cited articles appearing in lower-prestige journals...

... there are thousands of posters being fueled by highly caffeinated grad students and postdocs, which accounts...exhibit booths, happy hour socials, and late, late night socials. As a newbie to the SfN conference, I have...

...West African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement...links to the relevant scientific literature.  -In light of the current Ebola crisis, Science and Science...

...outcomes. For example, pd|hub assembled a coalition of higher education organizations committed to advancing ...educational path before we lose more scientists who might have played a major role in solving the world’s ...

...under the "Resource Information" heading in the right column of the plasmid page. Check out the purple...can also use the "Search Table" box at the upper right of the table to search and filter the list of citing...

... for academic research and training is getting tighter and competition is fierce. However, I believe there...conference is organized by postdocs for postdocs. I am delighted to see enterprising early career scientists taking...

...approaches. Why researchers should have a mix of high-risk, high-reward and low-risk but reliable projects. ...

...‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall. It ...addition to low costs, deep tissue penetration, and high spatial resolution are benefits of this technique...

... to make your own competent cells. If you need a high efficiency of transformation (for example, if you...However, you can skip this incubation step and go straight to plating if your transformed cells are being...

...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim...

...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ...

...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...

...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...

...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...

...what you're looking for. Search using the box highlighted in red above. When searching, it is worth trying...

...precise genome edits, the recombinases used must have high activity and specificity with low cross-reactivity...recombinase/reporter pairs were inactive, even at very high recombinase levels. These data show that multiple...

...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...

...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...