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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 281 - 300 of 960 results

  2. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... RNA degradation seen in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

  7. Plasmid Collections

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... our guides to find the plasmids and science information you need before starting your experiment. Genome...

  10. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this....org for more information and assistance with the deposit process. Want more information on the wide variety...publication or plasmid information page, or contact the depositing PI for more information on the experimental...publication associated with each plasmid for more information on how it was originally used. Here are a few...

  12. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee that ... our collection. The plasmids available in this format include a variety of popular plasmids and backbones...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by ...Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA order? For each cloning...DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful to requesting...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of... of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in one ...NeuroMabSeq platform. Mitchell et al. Sci Rep. 2023 Sep 27. See More Fluorescent Proteins Find information on ...has assembled the following to help you find information and plasmid collections that may help with your...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...Commun. 2023 Dec 5. See More Antibodies Find information on Addgene's collection of plasmid-based antibodies...Link opens in a new window) A news website and information resource dedicated to helping researchers accelerate...

  15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... random amino acid sequences or (ii) designed to form an inert, unstructured peptide and therefore consisted...by proteases. Nowadays, with ample structural information available, it’s clear that the C-terminus of ...the POI would work best. Ideally, structural information can guide this decision. In our experience, the...-Hermans et al., 2011).  Although structural information may guide design, it is advisable to generate...

  17. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... your manuscript is a form of (hopefully constructive) conflict. As you perform the experiments to address...trouble spots:  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization...

  18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...representation of integration process and episomal forms of non-integrating transgenes. Created with BioRender.com...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...

  19. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing cytoplasm of... at a distance that favors proper folding and formation of the antigen-binding site while also minimizing...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...challenge of bacterial expression is the proper formation of the disulfide bond between the VL and VH domains...of bacteria to allow for proper disulfide bond formation.  expression in strains of bacteria that are redox...Sandomenico et al., 2020). conversion to a cysteine free format that’s easier to express in the cytoplasm of E....scFvs designed for oncology applications are reformatted as bispecific molecules which bind CD3 and a ...

  20. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...restriction digestion to anneal to one another and form a complete, circular plasmid. You then add DNA ligase...You should perform, at minimum, two transformations after a ligation: 1. Control Transformation containing...contamination from uncut plasmid. 4. Transformation Transform your ligation reaction into your bacterial...Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of the restriction...Bromide Pre and Post UV Light 0.5 For more information on these stains see the Bitesize Bio Blog and... how many colonies you should expect in the transformation due to background re-circularization and contamination...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...
Showing: 281 - 300 of 960 results