We narrowed to 399 results for: MUT
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TypeBlog Post...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended...
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Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation consensus... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings of the... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... -
Five Popular Model Organisms, Part 2
TypeBlog Post...molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...process makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially... -
Plan Your Experiment
TypeCollection...knockout )? Generate a specific mutant allele of a gene ( point mutant )? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the... type or organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting... a particular gene, such as generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base...knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce expression... the N-terminus are targeted since frameshift mutations here increase the likelihood that a nonfunctional...of NHEJ. In HDR gene editing experiments, most mutated alleles will not contain the desired edit, as a... -
Deisseroth INTRSECT Collection
TypeCollection...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137119 pAAV-EF1a-Con/Fon-...Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55645 pAAV-hSyn Con/Fon hChR2...Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137148 pAAV-nEF-Con/Fon-Arch3.3...recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 177669 pAAV-nEF-Coff/Fon ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...molecularly evolved from mNeptune2 through 33 mutations and 2 deletions. CyOFP1 has a higher quantum yield... -
Hot Plasmids: Spring 2025
TypeBlog Post...designed and screened a large library of iGluSnFR3 mutants, identifying the two best-performing variants ... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies... -
Finding Your Perfect Job After University
TypeBlog Post...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ... -
COVID-19 Resources
TypeCollection...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive... and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning ...Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein RBD mutations for comprehensive...Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free access articles...Scanning Library - Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2... -
Transferable Skills Guide: Teamwork
TypeBlog Post...year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy. As it...