We narrowed to 922 results for: Por
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TypeBlog Post...grant proposals or to demonstrate impact in annual reports. While the raw data is, of course, interesting ...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...dashboard at-will with new data. These features are incorporated in the current dashboard. The dashboard is coded...
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Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog Post...nonprofit founded in 2007, strives to expand the opportunities and scientific resources available to researchers...facilitating connections to mentorship and networking opportunities, publishing assistance, and more to Instrumental...Seeding Labs, Addgene also feels it’s incredibly important for scientific breakthroughs, reagents, and know-how... -
Addgene Stands with the Scientific Community
TypeBlog Post... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this... -
Grad School Advice Part 2: Building Community
TypeBlog Post...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...you changed throughout graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post... - 18:29 : How did you hear about post grad opportunities and when did you start thinking about them? ... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...purifying a plasmid from a bacterial culture, it is important to consider your experiment. It will dictate ...To allow efficient and flawless cloning it is important that the preparation is pure from other nucleic...increase transfection success. In conclusion, it is important for you to establish in advance how you want to... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...smooth and simple as possible. We are the Customer Support Specialists here at Addgene. If you’ve called our...order? I tried searching my PO in the track order portal and nothing shows up. Where is my order? All of... store your plasmids or viral items, technical support, and more. ... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...stress and depression, and now researchers have reported a mechanism based on studies of transgenic mice... there is plenty more to explore. “It will be important to identify the key proteins and metabolites that...stress-induced depression. Agudelo LZ, Femenía T, Orhan F, Porsmyr-Palmertz M, Goiny M, Martinez-Redondo V, Correia... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...creating their own opportunities for career development and networking. Aside from the importance of the topics...transferable skills to enable pursuit of these diverse opportunities after training. One of the best recent discussions... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...at the Broad Institute and MIT published another report showing Cas9's structural details. "Understanding... -
Reflections and Looking Ahead
TypeBlog Post...We continue to expand our educational content supporting the tools in our collection, such as blogs describing...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...exactly what you need, faster. We’re also building a portal to easily share sequences and characterization ... -
Editor's Choice, September 2016
TypeBlog Post...I’m sitting in the San Francisco International Airport listening to the Lion King soundtrack and writing.... I love running and this event gives me the opportunity to dress up as Mr. Incredible, an added bonus...would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids on this... -
Management for Scientists: Seeking Feedback
TypeBlog Post...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ... Seeking feedback frequently One of the most important paths of information flow is the one that brings... -
Top 10 Open Science Developments of 2013
TypeBlog Post...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...sharing ideas for the company, but also talking about sports, movies, and just about anything. As our organization...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...easy as possible. However, I think it’s just as important to take a break from work to share time with the... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple important technologies... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success...takes us a decade or more for just the “training” portion of our careers. In that time we may have only 2... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...contribution of students in their labs and the importance of the strong, cross-disciplinary collaboration...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ... research in the scientific community and the importance of this type of experimentation to the eventual... -
9 Tips to Achieve Success in Academia
TypeBlog Post...Cepko 1. Work hard. There will be periods when opportunities arise. “Grab it!” 2. Don’t get caught up in ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying... -
Plasmids 101: What is a plasmid?
TypeBlog Post...components that make up a plasmid and why each is important. Our “Plasmids 101” series designed to educate...and selectively grown on antibiotic plates. Importantly, because the bacteria from which plasmids are...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share their...are both excellent ways to provide transparency, support reproducibility, and accelerate science. We encourage... as soon as the preprint is posted in order to support these goals. And we can update your plasmids’ citation... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...attendees an amazing opportunity to share their cutting edge science in increasingly important field. Interested...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the...29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy...functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit includes ...