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  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

  5. New Viral Vectors - Fall 2024

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    Blog Post
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

  7. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  9. Choosing a Good Mentor for Scientists

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    Blog Post
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  15. Antibodies 101: Epitope Tags

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    Blog Post
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  18. Pushing the Limits of DNA Assembly

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    Blog Post
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  19. 5 Great Apps for Lab Life

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    Blog Post
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  21. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  24. 25 Work From Home Tips for Scientists

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    Blog Post
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

  25. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  26. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  28. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  29. How to Lead a Great Meeting

    Type
    Blog Post
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

  30. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  32. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  33. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  35. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

  36. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

  37. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

  38. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

  41. dTAG - You're it!

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    Blog Post
    ...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

  42. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

  43. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

  44. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

  45. Addgene: The First Twenty Years

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    Blog Post
    ...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

  46. Summer SciComm: Preprints

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    Blog Post
    ... often post a preprint at the same time as, or slightly before, submitting the manuscript to a journal...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  47. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...1948 as a variation of the more commonly observed light emission by fluorescence. The widespread use of ...distances that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources...

  50. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...
Showing: 751 - 800 of 978 results