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  3. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

  4. MXS Chaining

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    Blog Post
    ...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...

  5. AAVs in Retinal Gene Therapy

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    Blog Post
    ...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  9. Summer SciComm Series: Modes of Communication

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    Blog Post
    ... work. After all, a stick-figure internet comic about math probably didn't sound like a great idea to ...  Selecting the method Once you’ve answered the above questions, you can create your short list of options...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post
    ...and detergents.  Organic Solvents  As mentioned above, organic solvents can be used to simultaneously ...permeabilizing with saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal...

  12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post
    ...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

  16. Plasmids 101: Gateway Cloning

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    Blog Post
    ... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

  17. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  19. How to Be an Excellent Trainee

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    Blog Post
    ...individuals who are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training...prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

  21. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

  22. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

  23. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...

  28. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

  30. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...

  31. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

  32. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

  33. Plasmids 101: Dimers and Multimers

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    Blog Post
    ...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...

  34. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...

  35. Quick Guide to All Things Lentivirus

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    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer...

  40. Typing CRISPR Systems

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    Blog Post
    ...Class 1 is the most abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly...CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions, and more...

  41. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion editors...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing...

  42. Antibody Validation for Flow Cytometry

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    Blog Post
    ...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

  43. Delivery Methods for Generating iPSCs

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    Blog Post
    ...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

  45. New Antibody Animation!

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    Blog Post
    ...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

  47. Plasmids 101 eBook 4th Edition

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    Blog Post
    ...take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they go to...

  49. Antibodies 101 Series on TikTok

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    Blog Post
    ... is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies animation has all...
Showing: 751 - 800 of 962 results