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TypeBlog Post...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...
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The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using... -
Targeted Mutagenesis with EvolvR
TypeBlog Post... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post.... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India Devarshi Gajjar has ... -
Antibodies 101: ChIP
TypeBlog Post...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those... -
Ras Pathway
TypeCollection...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed... -
Control AAV Preps
TypeCollection... 1, 2, 5, 8, 9, rg*, PHP.eB Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...PubMed Central PMCID: PMC49545. 3. Coffin J, Hughes S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed... -
All in a Twist: dsRNA
TypeBlog Post...1414 (2018). 10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and ... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators... -
Hot Plasmids - February 2022
TypeBlog Post...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...edition. References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...