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TypeBlog Post...DNA repair. A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...
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Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using... -
Targeted Mutagenesis with EvolvR
TypeBlog Post... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297... -
Antibodies 101: ChIP
TypeBlog Post...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post.... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India Devarshi Gajjar has ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...edition. References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide... -
Hot Plasmids - February 2022
TypeBlog Post...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...PubMed Central PMCID: PMC49545. 3. Coffin J, Hughes S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed... -
All in a Twist: dsRNA
TypeBlog Post...1414 (2018). 10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and ... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...