We narrowed to 344 results for: ADA

1. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...

2. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   ...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...

3. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   ...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

4. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

5. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   ...smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes for light and ...

6. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

7. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   ...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...

9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

10. Plasmids 101: Biotinylation

    Type

    Blog Post

    .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

13. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

14. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

18. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

19. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...

20. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated...