We narrowed to 339 results for: ADA

1. Antibiotic Resistance: An Old Solution but a New Problem

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   Blog Post

   ...bacteriology and drug resistance.  It seems as though, nowadays, we cannot make it a full month without a new,...

2. Using Video to Share Your Science: We Share Science

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   Blog Post

   ...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...

3. Plasmids 101: Codon usage bias

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   Blog Post

   ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

4. CRISPR Activation: A Practical Guide

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   Blog Post

   ...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   ...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...

6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

7. Plasmids 101: Biotinylation

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   .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

8. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

9. Five Popular Model Organisms, Part 2

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   Blog Post

   ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

11. Quick Guide to Near-Infrared Fluorescent Proteins

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    ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

12. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

14. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

15. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Widrick, J. J., Messemer, K. A., Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz...

17. Viral Vectors 101: Optogenetic Tools

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    ...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...

18. Viral Vectors 101: AAV Variables That Matter

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    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated...

19. Antibodies 101: Multiplex Immunofluorescence

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    ...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...

20. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    .... Jonkers, M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for ...