We narrowed to 382 results for: Bre;

1. Common Injection Routes in Mice

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   ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

2. Twenty Years of Addgene Sharing: CRISPR

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   ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

3. 3D Printing Meets CRISPR Cas9

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   ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

4. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

5. PhD Applications After COVID

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   ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

6. Polymerase Chain Reaction Overview and Applications

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   ...dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the separation...

7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

8. Transferable Skills Guide: Managing a Team

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   ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

9. Truncated gRNAs for Regulating Gene Expression

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   ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

11. Experimenting with New Careers while in Grad School

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    ...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

12. Genome-wide Screening Using CRISPR

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    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

13. Light Sheet Fluorescence Microscopy

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    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data...

15. Which Fluorescent Protein Should I Use?

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    ...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

16. Neurodegeneration Plasmid Collection

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    ...TEV-HsFbxo7-129-398/Skp1 FBXO8 GST, TEV tac Parkinson's Brenda Schulman 203365 Tau BA 12->7 K257T MAPT Flag CMV...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...

17. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios...

18. Great Results Start with Great Standard Curves

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    ...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...

19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

20. AAVs in Retinal Gene Therapy

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    .... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...