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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 651 results for: CHI

Showing: 301 - 320 of 651 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...

  3. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in...

  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

  6. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

  8. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

  11. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  17. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

  18. Advice for Choosing a Research Project

    Type
    Blog Post
    ...PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important factors to consider...months reading and writing a proposal before even touching a pipetteman. Take the time to generate new ideas...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...
Showing: 301 - 320 of 651 results