We narrowed to 314 results for: Cas9
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TypeCollection...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...
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The 12 Days of CRISPR
TypeBlog Post...🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in... -
Neurodegeneration Plasmid Collection
TypeCollection...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...editing in moderate thermophiles. These evolved SpCas9 variants have improved activity on NAG PAMS and... -
CRISPR Plasmids - Epigenetics
TypeCollection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post...comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables... -
CRISPR Plasmids - RNA Editing
TypeCollection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...