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We narrowed to 570 results for: Cit

Showing: 301 - 320 of 570 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... make your ideas easier to understand and more exciting. Practice presenting your chalk talk Perfect your...research program. You should be proud of it and be excited about getting the opportunity to realize your vision...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to... future post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type ...

  8. Optogenetics Guide

    Type
    Guide
    ...Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific cation influx...physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic ... as light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements...are some key factors to consider: Optogenetic excitation or optogenetic inhibition. First things first...experiment? Depending on your answer, you’d pick an excitatory or inhibitory opsin, respectively. Color of activation... EJ, Tian Z, et al. 2014. Independent optical excitation of distinct neural populations. Nat Methods. ...Finkelstein J, Paz JT, et al. 2011. Neocortical excitation/inhibition balance in information processing ...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...and the fluorescence signal weakens. (b) The heterogenicity of proliferative behavior in a cell population...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Overall, the synthetic rAbs demonstrate remarkable specificity for their targeted integrin subtype, even when... with the Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered...

  11. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...decks leads to the opposite and can suffocate an exciting idea in a swamp of unnecessary details. You DO...

  12. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or break your...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...

  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Proteins can also be biotinylated by exploiting the specificity of the E.coli BPL, BirA. If you recall, BirA ...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b...

  15. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins...the reaction, or have difficulties with kinase specificity in vitro. The establishment of a system in which...

  18. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...DNA synthesis but further limits the packaging capacity of rAAV vectors to ~2.4 kb. Figure 4: Examples...ability to target specific tissues and cells, and capacity for long-term gene expression. These features ...other viral delivery systems due to their non-pathogenicity, ability to efficiently deliver genes to a wide...brain structure due to their small size, low immunogenicity, and limited ability to spread after injection...capsid is challenging due to its limited packaging capacity, especially if any additional regulatory elements... information. Split AAVs to increase packaging capacity Another strategy to deliver cargo too large to...Engelhardt, J. F. (2001). Expanding AAV packaging capacity with trans-splicing or overlapping vectors: a ...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...
Showing: 301 - 320 of 570 results