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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 572 results for: Cit

Showing: 301 - 320 of 572 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

  2. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to... future post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type ...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Proteins can also be biotinylated by exploiting the specificity of the E.coli BPL, BirA. If you recall, BirA ...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b...

  6. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...

  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins...the reaction, or have difficulties with kinase specificity in vitro. The establishment of a system in which...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...and the fluorescence signal weakens. (b) The heterogenicity of proliferative behavior in a cell population...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Overall, the synthetic rAbs demonstrate remarkable specificity for their targeted integrin subtype, even when... with the Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...

  12. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...decks leads to the opposite and can suffocate an exciting idea in a swamp of unnecessary details. You DO...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or break your...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

  15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...type depends largely on the viral capsid, but specificity doesn’t end there: expression from rAAVs also...Transduction but Differ in Sialic Acid Linkage Specificity. Journal of Virology, 75(15), 6884–6893. https...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9... in order to call that gene a hit.  This is an exciting time for functional genomics, with an ever-expanding...

  18. Finding Your Perfect Job After University

    Type
    Blog Post
    ...treatments for external companies. It was really exciting to work in a larger commercial company; the pay...opportunity to work in a genetics lab. As a UK citizen, I was entitled to a Work-Travel VISA, which means...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...shown to offer high activity and unparalleled specificity in genomic targeting design flexibility. The ...cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently ...
Showing: 301 - 320 of 572 results