We narrowed to 768 results for: Dos
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TypeBlog Post...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...
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SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...using microfluidic devices. Addgene: What other uses do you foresee for this tool? Howarth: Polyproteins ...infected with malaria or other parasites. Addgene: Do you have any particular tips or tricks for those ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won... -
Advice for Choosing a Research Project
TypeBlog Post...experiments and why? What techniques and skills do you need to use to reach these milestones? Can you...milestones along the way so that you don't get stuck doing the same experiment for too long. Keep evaluating... -
9 Tips to Achieve Success in Academia
TypeBlog Post... yourself with this passion. “Love what you are doing!” It helps when the time comes to work with full...become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside of... -
Arf GTPase Family
TypeCollection...opens in a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should... -
Plasmids 101: Codon usage bias
TypeBlog Post... preferences and what can we do about them? Read on to find out! Why do organisms have different codon...necessarily mean the gene is going to express well. If you do get good expression, you should also functionally... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came ...? The AbuGoot lab realized that they could likely do it using Prime editing alongside some creative (and...represent a major step forward for what the field can do. References and Resources References Özcan A...integrases. Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post... the school district itself in terms of what they do with their discretionary money. They also usually...for any topic--not just CRISPR): Who am I? Where do I work? The teachers emphasized that some students...and other science communicators. What can CRISPR do? I spent more time on this section of the presentation... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post... team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent protein...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made... MagLOV raise broader questions like “How and why do fluorescent proteins respond to magnetic fields?”... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...staining Can be expensive Nanobodies Recombinant; do not require animal sacrifice Good tissue penetration...Unlike large secondary antibodies, small nanobodies do not oligomerize with primary antibodies when incubated... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... out of the park. It’s clear that you know how to do the job. You may think this means you’re guaranteed...to thrive at the company and add value, not just “do the job”. When they ask about your creativity, your...good but focused on all the other things you have to do. Pause! Enjoy that feedback, bask in the glow of ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...in our lab’s environmental impact, but we can all do more to make research more environmentally friendly...recycling program at your institute! What should I do in the lab to help the environment? Well, you could... -
Plasmids 101: What is a plasmid?
TypeBlog Post...plasmid to manipulate and use in later work. How do scientists use plasmids? Generally, scientists use...Some of the many things that plasmids can be used to do include: Produce large amounts of a protein so that... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post... time a post doc in the Quake Lab, volunteered to do the sampling and I happily agreed to accompany him...temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...call out every Addgene Team because they’re all doing amazing work during this difficult time. One function...distributes and fulfills requests for scientists doing almost every type of biomedical science research... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with... but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making... -
Intro to Our Summer SciComm Series!
TypeBlog Post...at a peer audience. Note that lay people can and do consume scientific communication (it is, after all...moves from layperson to expert) and peers can and do consume science communication. Formal education ... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...edible relatives, it is fair to say that most people do not recognize its importance or perhaps even know...Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are you a biology... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...are not enough to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA... just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR Genome Editing...