...you may not have the chance to stop what you’re doing and make a phone call or write out an email. You...which you can describe your issue and press send. Doing so will bring your concern directly to our support...

...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...

...using microfluidic devices. Addgene: What other uses do you foresee for this tool? Howarth: Polyproteins ...infected with malaria or other parasites. Addgene: Do you have any particular tips or tricks for those ...

...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won...

...experiments and why? What techniques and skills do you need to use to reach these milestones? Can you...milestones along the way so that you don't get stuck doing the same experiment for too long. Keep evaluating...

... yourself with this passion. “Love what you are doing!” It helps when the time comes to work with full...become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside of...

...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...staining Can be expensive Nanobodies   Recombinant; do not require animal sacrifice Good tissue penetration...Unlike large secondary antibodies, small nanobodies do not oligomerize with primary antibodies when incubated...

... out of the park. It’s clear that you know how to do the job. You may think this means you’re guaranteed...to thrive at the company and add value, not just “do the job”. When they ask about your creativity, your...good but focused on all the other things you have to do. Pause! Enjoy that feedback, bask in the glow of ...

... the school district itself in terms of what they do with their discretionary money. They also usually...for any topic--not just CRISPR): Who am I? Where do I work? The teachers emphasized that some students...and other science communicators.  What can CRISPR do? I spent more time on this section of the presentation...

...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came ...? The AbuGoot lab realized that they could likely do it using Prime editing alongside some creative (and...represent a major step forward for what the field can do.    References and Resources References  Özcan A...integrases.   Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells...

... team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent protein...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made... MagLOV raise broader questions like “How and why do fluorescent proteins respond to magnetic fields?”...

... preferences and what can we do about them? Read on to find out!   Why do organisms have different codon...necessarily mean the gene is going to express well. If you do get good expression, you should also functionally...

... single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should...

...Biology. 2010 Feb;2(2):a001107. PMC PMID: 20182618 . Do you have suggestions for other plasmids that should...

... FRET) with pmVenus(L68V)-mTurquoise2 Back to Top Do you have suggestions for other plasmids that should...

...edible relatives, it is fair to say that most people do not recognize its importance or perhaps even know...Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are you a biology...

...are not enough to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA... just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR Genome Editing...

...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript ...introduction, etc. so as to thoroughly explain the do’s and don’ts of that week’s piece of a manuscript....

...call out every Addgene Team because they’re all doing amazing work during this difficult time. One function...distributes and fulfills requests for scientists doing almost every type of biomedical science research...

...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with... but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making...