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We narrowed to 771 results for: Dos;

Showing: 301 - 320 of 771 results

  2. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won...

  3. Advice for Choosing a Research Project

    Type
    Blog Post
    ...experiments and why? What techniques and skills do you need to use to reach these milestones? Can you...milestones along the way so that you don't get stuck doing the same experiment for too long. Keep evaluating...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... yourself with this passion. “Love what you are doing!” It helps when the time comes to work with full...become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside of...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s to be used...have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... the school district itself in terms of what they do with their discretionary money. They also usually...for any topic--not just CRISPR): Who am I? Where do I work? The teachers emphasized that some students...and other science communicators.  What can CRISPR do? I spent more time on this section of the presentation...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... preferences and what can we do about them? Read on to find out!   Why do organisms have different codon...necessarily mean the gene is going to express well. If you do get good expression, you should also functionally...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...staining Can be expensive Nanobodies   Recombinant; do not require animal sacrifice Good tissue penetration...Unlike large secondary antibodies, small nanobodies do not oligomerize with primary antibodies when incubated...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ... out of the park. It’s clear that you know how to do the job. You may think this means you’re guaranteed...to thrive at the company and add value, not just “do the job”. When they ask about your creativity, your...good but focused on all the other things you have to do. Pause! Enjoy that feedback, bask in the glow of ...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came ...? The AbuGoot lab realized that they could likely do it using Prime editing alongside some creative (and...represent a major step forward for what the field can do.    References and Resources References  Özcan A...integrases.   Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells...

  12. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure...absorbance readings are inherently more variable. If you do need to work with dilute samples:  Take multiple...the references we have provided below. And if you do have trouble with contamination, feel free to check...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent protein...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made... MagLOV raise broader questions like “How and why do fluorescent proteins respond to magnetic fields?”...

  14. Arf GTPase Family

    Type
    Collection
    ...opens in a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should...

  16. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...in our lab’s environmental impact, but we can all do more to make research more environmentally friendly...recycling program at your institute! What should I do in the lab to help the environment? Well, you could...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with... but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making...
Showing: 301 - 320 of 771 results