We narrowed to 821 results for: EED

...location and type of edit you need to introduce highly specific? Perhaps you need to introduce a single nucleotide... have little flexibility as to where your sgRNA needs to be located and there may not be a NGG PAM site... and published in peer reviewed journals. If you need to generate a new sgRNA for your experiment, select...

...antibody host species and isotypes. Consequently, users need to choose the protein type that is most compatible...those in the nucleus, more stringent buffers may be needed. Since many factors, including salt concentration...strength and pH, affect binding the lysis buffer may need to be adjusted depending on the sample. It is important...be translatable to the endogenous system and will need to be verified using alternative methods.  Finally...

...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...Because rabies dG can’t infect neurons on its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA...pseudotyping that could be broad or specific to suit your needs. Check out some of these review articles to find...https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ...

...updated pEcCas/pEcgRNA system, and confirmed that indeed the gRNA-pMB1 had a slightly higher leaky expression...MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids (pDonor and pQCascade... strain library, our pEcCas/pEcgRNA system would need to construct 13 plasmids with 14 rounds of transformation...of these systems a try, we’d love to hear your feedback!   Thank you to our guest bloggers! Qi Li obtained...

... modify the protocol to best fit their specific needs. Herein, we’ll give you a rundown of the different...directly conjugated to the reporter, there is no need for a secondary antibody incubation step, saving... the signal. Since the primary antibody does not need to be conjugated, and conjugated secondary antibodies...feel empowered to choose the best ELISA for your needs. Good luck and happy binding! More resources on...

...initially created to support the CRISPR analysis needs of Synthego’s scientists, and it’s now free for ...experiments and comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE...ICE will do the rest. There are no parameters that need optimizing and no complicated steps to learn. For...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...

... given set of resources, time, and experimental needs. Since its invention in the late 1990s, Gateway ...easily select for the expression clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. ...clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation of the...set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products directly...

...of codons, it only needs to produce a subset of charged tRNAs and therefore may need fewer resources for...Adaptation to changing conditions - Organisms often need to express genes at different levels under different...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

...issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed raising the salary...for Dec 1 2016. In order to be exempted from the need to pay employees overtime, salaries must be above... of $23,660. Any postdocs on such a salary would need to have their salary raised 101% if hours are not...make gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a...

...reduce non-specific binding of antibodies.  You may need to optimize the type of block used, the concentration...which will bind to your target molecule. You may need to optimize the concentration and incubation time...antibody and is conjugated to a fluorophore. You may need to optimize the concentration and incubation time...antibody seems and what sort of protocol you will need to use. (To learn more about antibody validation...

...mature sequence in a plasmid and call it a day. You need to include both sides of the complementary RNA duplex...hairpin loop sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are...issue with many gene silencing methods and just needs to be taken into account when designing experiments...of study for improving these tools. Next time you need to knock down a gene, maybe you’ll consider using...

...organism; they are relatively simple to maintain and breed and they have large, easily modifiable embryos, ...frog research is The National Xenopus Resource, a breeding facility for X. laevis and X. tropicalis, the ...development, a large number of offspring (produces many seeds), self fertilizing, a small yet highly characterized...Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms. Dong wu xue yan...

...scientists as well as other citizens. Thus civilization proceeds in an informed fashion. For science to be useful...representative in the House of Representatives all you need to know is your area code. Senators: To find contact...contact information for your senators all you need to do is select your state. State Legislatures: To find...Trends in Federal R&D NJ.com: Rush Holt: Lawmakers need to use a scientific approach to formulating views...

...undesired mutations through the PCR process, you need a high-fidelity polymerase. There are many high ...high fidelty polymerases on the market; you need one with 5’->3’ polymerase activity (for amplification),...potentially truncate the template). Importantly, you also need a DNA polymerase that produces blunt-ended PCR products...DNA duplex stability from the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986...

...fluorometric-based detection. This increased sequencing speed ultimately leading to what are generally considered...became the workhorses of molecular biology." Proceedings of the National Academy of Sciences 102.17 (2005...restriction endonuclease of Hemophilus influenzae." Proceedings of the National Academy of Sciences 68.12 (1971...sequencing with chain-terminating inhibitors." Proceedings of the National Academy of Sciences 74.12 (1977...

...choice as shown in the figure below. To start, you need to have DNA fragments with regions of homology at...step without scars as depicted below. First, you need to design primers to amplify the two fragments while...is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding ...fragments. CRISPR cutting was used to circumvent the need to PCR amplify the fragments of the chromosome, ...

...previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works...provided neuroscientists with the vast array of colors needed to mark individual neurons, the system also suffered...distinct cell populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes...provides spatial and temporal control without the need for germline modification, and enables Brainbow ...

...off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue. Above we mentioned...You only successfully generate 1 null clone and proceed with it. In this case, the likelihood of having...factors. Hopefully now you have all the resources you need to determine what your risk level is, how to lower...

... would breed them many times, hoping to produce a mouse with the desired edit. You’d also need to remove...remove the selection marker, likely by breeding to a mouse line with a recombinase. If it turned out your...design a knock-in allele at a given location, you’ll need to design only a single guide and your donor ssDNA...

... proteins with host-binding activity without the need for genome sequencing or annotation (5). In the ... shearing exist as well, such as sonication and needle shearing. Essentially, any method that results ...forceps. Several protein induction conditions may need to be tested, as with any protein purification, ...DNA fragments with live host cells Now you can proceed with incubating this membrane with your cells of...