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We narrowed to 629 results for: EMB

Showing: 301 - 320 of 629 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...committee in graduate school. Thesis committee members provide expertise in designing and interpreting...to introduce yourself to and interact with the members of your field, and present your research. Face ...family. Those people are your support network, so remember to thank them verbally and often for their love...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...164084 Knockout Human Sabatini 3rd 10 7,501 Human Membrane Protein Activation Library 113345 Activation Human...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody recognizing the protein of...is added, and will bind to that protein on the membrane. Then, addition of a secondary antibody allows...bind to proteins on the cell surface, or the cell membrane can be permeabilized to allow antibodies to bind...

  4. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore, certain...detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment,...dynamic samples Ex: 3D cell cultures, small model embryos >15 μm Thsee are some of the most challenging samples...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... in CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak,...CTD and the entire REC lobe—do not structurally resemble any other known proteins; they are completely ...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...single stranded, negative sense RNA virus. It’s a member of the Paramyxoviridae family of viruses, which...staining for alkaline phosphatase, expression of embryonic proteins, and promoter demethylation and expression.... Cells can be differentiated in vitro to form embryoid bodies which are compact balls of loosely organized...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... Biology 22:169–174 . https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A...Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher...Chem Biol 13:443–448 . https://doi.org/10.1021/acschembio.7b00603 Additional Resources “Optogenetics Meets...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...1), which may be easier to conceptualize. Just remember to switch them back before reporting the data!...use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon statistical laxness...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

  10. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...It's December, and that means it's officially the start of Addgene's winter holiday celebrations! We'...with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew Norton and NEBGCE4All...

  11. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ... former Co-director of Science in the News, and member of the SITN podcast team.     Amy Gilson recently...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  12. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday...presented in this blog post includes data until November 9, 2020. Between then and the posting of the blog...

  13. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ... that everything will ship out in time - from assembling the boxes, to inoculating tubes, and to packing...pic.twitter.com/eJbI0lLiGQ — Dr. Joanne Kamens (@JKamens) September 13, 2018 This massive scale reagent sharing led...

  16. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and programming with...great European photo slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...are coming along at a fast and furious pace. In December, as we've blogged about before, there was the ...via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) ...
Showing: 301 - 320 of 629 results