We narrowed to 438 results for: ENA;

1. Antibodies 101: ChIP

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   Blog Post

   ...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

2. Hot Plasmids - February 2022

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   Blog Post

   ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...

3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...

4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back...

6. AAV Q&A with Tim Miles

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   Blog Post

   ...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...

7. CRISPR 101: Non-Homologous End Joining

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   ...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

8. Plasmids 101: Yeast Vectors

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   Blog Post

   ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether...

11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    ...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...

12. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    ...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

13. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome...

14. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...antigen tend to work very well for applications that denature a protein to its primary (linear) structure using...

15. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further ...

17. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

18. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1...

19. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ...specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian ...

20. The Future of Research Symposium Boston 2015

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    Blog Post

    ... of the biomedical research sector, and what a tenable workforce structure may look like. The panel discussion...