We narrowed to 447 results for: ENA;
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TypeBlog Post...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back...
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Targeted Mutagenesis with EvolvR
TypeBlog Post...Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA... -
How Addgene Creates Content
TypeBlog Post...sharing speeds science, and our entire mission is to enable that process. Our repository of plasmids, AAVs,... -
AAV Q&A with Tim Miles
TypeBlog Post...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split... -
Antibodies 101: ChIP
TypeBlog Post...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount... -
Hot Plasmids - February 2022
TypeBlog Post...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...lasers, with two-photon excitation at 1030 nm, enabling two-photon imaging without compromising on brightness... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...Luminescent Imaging with Nano-lanternsĀ Technologies Enabled by NanoLucĀ® Luciferase Fluorescent Proteins 101... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...a virus can infect. The VSV-G envelope protein enables this virus to infect many different cell types ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson... -
Plasmids 101: Yeast Vectors
TypeBlog Post... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a... -
Visualizing Protein Turnover In Situ
TypeBlog Post...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...