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Showing: 301 - 320 of 711 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...promoters can be used in the repressible manner described above, as well as the inducible manner detailed...30 years since the original Tet-Off system was described by Gossen and Bujard, both the promoter and transactivator...cells, and can be used in the repressible manner described above, as well as the inducible manner detailed...

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...for and how can you do it well? Below, I briefly describe some tips for a successful postdoc fellowship.... there a tractable question or are you simply describing a phenomenon? Exploration can be important, but...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

  3. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... been described in this blog. If your data does not meet the assumptions for the tests described above...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... community is generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed...videos and text on the website for more detailed descriptions of how to use the software. Here is a brief ...sequences, and more. Output_Compact: A text file that describes which genes were included, and how many gRNAs ...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease...

  6. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com/pYBL3aLK4f...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

  8. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

  9. Editor's Choice, July 2016

    Type
    Blog Post
    ...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments...

  11. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

  14. Editor's Choice, September 2016

    Type
    Blog Post
    ...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for...

  15. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...

  17. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...
Showing: 301 - 320 of 711 results