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We narrowed to 765 results for: Mos

Showing: 301 - 320 of 765 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...contain several important elements, but among the most important are the inverted terminal repeats (ITRs...modifications and types  Since ITRs are one of the most important elements of AAV and rAAV vectors, modifications...T-shaped hairpin loop sequences (Wilmott et al. 2019). Most ITR sequences are from AAV2 because they demonstrate...

  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...cell differentiation fate to tumor suppression (and most everything in between), understanding how, when,...selections In its current format, the MORF library is most immediately useful for TF library screens. Previously... More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...of heat-inducible lentiviral CAR constructs, the most basic of which is depicted here: The heat-inducible...and, as you might suspect, it has been one of our most commonly requested antibodies. But sometimes, one...temperatures, and other chaotropic conditions better than most FPs. The group also tested hfYFP in expansion microscopy...

  4. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...fluorescent proteins (FPs) have become one of the most indispensable tools in modern biological research...probe (be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment...soon as possible after submission. Using FPbase The most obvious use case for FPbase is finding information...

  5. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ... plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified...protocol, but the overall workflow is similar for most libraries. Here are the key points you’ll need to...Figure 3A). Addgene finds that this representation is most easily understood visually. As an example, the curves...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ..., including dendritic and axonal extensions and, most importantly, any connections the neurons make to...capable of spreading across synaptic connections. Most famous is the Rabies Virus (RABV) which moves retrogradely...  Commonly used serotypes include AAV1 – 9, with most of these serotypes capable of entering neurons (...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res...

  7. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ... write them on a board to see which ones get the most hits. Commit to a meeting schedule and meet regularly...ready for the discussion with resources. One of the most common questions I get is, “What are we going to...– compete for fastest to set up a coffee date or most cards collected. The next blog in this series will...

  8. Clear and Accessible Writing

    Type
    Blog Post
    ...Addgene’s content team, we — Jo, Brook, and Angela — most often think about accessibility in terms of the ...working together to share information. Sometimes it is most helpful to use a lot of jargon and have a technical...takeaway.  In this image, we thought it would be most helpful to highlight the technical details of collecting...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...injected 1e+13 vg per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    ...each other until you are ready to separate them. Most proteins are not likely to remain attached to the...want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding can ...and complex, you may be thinking, well, these are mostly fairly standard molecular and cell biology techniques...

  11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...? There have been several systems to do this but most are labor-intensive, require multiple fluorescent...They continued to use GEmCherry2 to identify the most efficient sgRNAs to target a specific locus to create...efficiency. So if you are looking to identify the most effective gRNA and enrich cells for your CRISPR ...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...classroom cannot offer. Furthermore, companies across most industries tend to hire their former interns to ...assess your eligibility, interest, and career fit. Most internships share a common set of criteria such ...background, and lighting for a smooth performance. Most importantly, make certain that everyone you live...

  13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...genetic modification by homologous recombination. In most recombineering strategies, only 3-4 phage proteins...a whopping 144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such... which are recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...requested in the call. Step 2: Develop a research plan Most grant applications restrict the space available ...more advice on writing a grant proposal. "Murder Most Foul: How Not to Kill a Grant Application" by Vid...process? What types of writing feedback do you find most helpful? Have a great grant writing tip? Share it...

  15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is...resistant to CcdB, as the native ccdA will be present. Most common cloning strains of E. coli do not contain...ever more important to ensure that you choose the most appropriate method for your own experiments. Please...

  16. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

  17. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary antibodies you ...antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain...convenient site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have...

  18. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...systems, making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be...compact genome editors and a gRNA down-sized by almost 40%. The activity of Cas12f1 with these modified...of the system to optimize its outcomes. In their most recent work, the Liu Lab boosts the efficiency of...

  19. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...facilitate its detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence...fluorescent protein (GFP) and mCherry, two of the most common tags currently used, are approximately 240...larger proportion on the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags...

  20. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... expression, you are almost certainly working with an endogenous locus. The most straightforward way to...function at the post-translational level, unlike most of the other mechanisms described. Fig. ...
Showing: 301 - 320 of 765 results