We narrowed to 928 results for: Mpl

...interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins) allow simultaneous...properties or trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins...needed, the use of HA and other short epitope tags (Kimple et al., 2013) could help improve the transport ...mCherry (top) or HA tag (bottom). The images show examples of dendrites (blue shades) in the monkey amygdala... not generalizable to all fusion proteins. For example, we saw that another type of DREADD, hM3Dq, is ...Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope...50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL, Pasker RL (2013) Overview of Affinity...

...track of past accomplishments. Follow all the rules of good resume writing. Include examples of concrete.... When I am speaking at a new institution, for example, I will often see a whole bunch of notifications...about (or how good the beer was at that place, for example). This is also a great way to keep track of how... and trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking...’m asked a lot about requesting connections to complete strangers for informational interviews. Many have...concrete accomplishments in both the technical and “plus skills” areas. Include a professional picture. It doesn...

... do as genetic circuits become larger and more complex? Voigt: If you look at all the products of biology... on genes at different times. It requires more complex synthetic networks. Addgene: What hurdles did you... perspective and you can layer them for more complicated functions. Those things are easy with CRISPR..... There haven’t been enough genetic parts for complexity. We have characterized almost 600 terminators...of a cell. The objective is to give people the complete toolbox for taking on very large genetic engineering...biotechnology today are things like butanol - very simple molecules - a few carbons and oxygens. Nothing ...just using what biology has given us. Wood is a complicated material and we can access it, but we are stuck...

...Although these multi-component systems can be complicated to implement, they can be highly specific with a small...Various Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications...ligands to the same sample to label sub-populations of your protein of interest. For example, the Cohen lab... assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled..., biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate synthetic...

...VPR fuses a tripartite complex with dCas9 to activate transcription. This complex consists of the VP64 ...CRISPR/Cas systems, however, greatly improved the simplicity of gene activation: rather than requiring protein...sequences, CRISPR/Cas systems rely on a guide RNA complementary to the sequence of interest. Upon binding, CRISPRa...CRISPR activator. While it requires a relatively simple construct, it exhibits modest levels of gene activation...transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

...binding site (PBS) complementary to the genome and a reverse transcriptase (RT) template including the desired...reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates, it is even more...primer binding site (PBS) and reverse transcriptase template (RTT). Created with BioRender.com. The first...encourage the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...

...toxins are the clearest example of a plasmid benefiting its host. For example, cells with a penicillin...genes between bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ...Shareck et al., 2004). Plasmid categorization is complex Plasmid classification has shifted over the years...Plasmids may also have more than one function: for example, Gateway cloning relies on genes found on an E....plasmids without clear analogues in nature. For example, helper plasmids were developed to replace helper...

... SCF E3 ubiquitin ligase complex. In the presence of auxin, this TIR1 complex binds to the degron sequence...assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms of its sequencing, in vivo studies...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector...assembly (backbones available at Addgene) with examples of potential synthetic chromatin mediators and...and should be varied as it can have important implications for the fusion protein effectivity), and a fluorescent... developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until it'...

...progression of your work, and you can accomplish this by employing a neat and organized format.  Columns...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...The UCLA Library offers some Powerpoint based templates with different column numbers for a good starting...starting point. Adobe also offers free poster templates, although most are vertical.  Large central box...Another commonly used and effective style is one employing a large central box, often used for displaying...

...connections between different brain regions. For example, communication between the hippocampus and the ...dendrites of proximal neuronal cells. In the specific example of episodic memory, a subset of hippocampal neurons...it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...that express Cre in only specific cell types, for example only dopaminergic neurons, neuronal tracing has...unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors is the Allen...part of the brain in a less invasive manner . For example, in neuromuscular diseases it would be desirable...

...thought of as descendants of totipotent cells. An example of pluripotent cells is embryonic stem cells (ESCs...lineage. Blood (hematopoietic) stem cells are a good example of multipotent cells. Unipotency: As the name suggests... mostly from abnormal and/or supernumerary preimplantation embryos that are produced during in vitro fertilization...maintain cell numbers or replenish dying cells. For example, hematopoietic stem cells found in the bone marrow...in science communication and publishing after completing her postdoctoral training from Massachusetts ...Extraction of Pluripotent Embryonic Stem Cells: Implications for Regenerative Medicine and Reproductive Medicine...embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis. Hum Reprod, 2008. 23(1):...

...colleagues. Here you simply PCR-amplify your vector and your insert, DpnI digest away the templates, and transform...will require a PCR reaction or two to generate templates for the cloning process. Time spent actually doing...relatively new to the cloning game, however, or simply looking for a more streamlined approach, you might...Golden Gate assembly allows one to simultaneously complete an ordered set of scarless ligations following...1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent...

... et al., 2015). In these studies, the strategy employed was to deliver a standard AdV vector to the targeted...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...genes. For this you would need to provide a DNA template to repair the double strand break via homology...Cas9/sgRNA and one vector for your specific DNA template. Using this two vectors system, Manuel Gonçalves...a potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...tools can also be used to deliver Cas9 and DNA templates in somatic cells of adult animals in order to ...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

...some simple solutions available for researchers to deal with all of these problems. The simplest and best...forming dimers in cells. Why should you care? Three simple ways a dimeric FP could ruin your day (and experiment...cell. The Kd of EGFP is 0.11 mM [2]. Following the simple logic outlined above, you might think EGFP is unlikely... a cell. Unfortunately, things are not quite so simple. Concentration is the number of molecules within...around that fusion protein is very high. Real world examples of these situations in cells are described below...form heterodimers (i.e. EGFP and mCherry). These simple strategies can help you make better fluorescent...

...and replaced it with a double-bonded oxygen. This simple enzymatic reaction enabled conversion between the...and one-ring pyrimidine base. This is a far more complex chemical task, not a job for a single enzyme. ...bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence, and (3) a protospacer-adjacent...separate and preventing repair based on the complementary strand. This could explain why the edited U ...original G is not primarily converted back to a complementary C. Optimized base editing with eOPTI-CGBE and...so the Yidi Sun and Erwei Zuo labs tested and implemented several of these advances for CGBEs (Yuan et ...replaces a target region with a newly synthesized, templated sequence. And the story still isn’t over! Keep...

...many biological fields. In synthetic biology for example, high-throughput cloning can be used to combine...one such technique and has been used to produce complex fluorescent reporter constructs. These fluorescent... variety of other clever applications. In one example, modular constructs generated with MXS-chaining...being directional, meaning that (in the previous example) one can control whether the third module is added...various cloning methods are listed in Table 2. For example,  chaining-based cloning methods require that the...general types of modules can be assembled. For example, because CpG dinucleotides are underrepresented...these types of constructs robustly, quickly, and simply. We hope that you can similarly apply MXS chaining...

...the other screen, implying that ORF and CRISPRa libraries can be used in complement to study gene function... stranded DNA breaks that commonly result in a complete loss of function while nuclease-deactivated Cas9... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...have several limitations. CRISPRko results in a complete loss of function meaning that if an essential ...transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): 583. PubMed PMID: 25494202...et al. "A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

...his program. Initially, Provost made his labs completely open-ended, allowing every student to work on... the culmination of many such experiences. For example, students in Provost’s course recently used a combination...Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased students’ confidence...Community (MCC) that seeks to reduce barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell...for your classroom, I highly recommend you try implementing one. It's likely only a matter of time before...Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance... Kowalski, J., Hoops, G., Johnson, J. 2016. Implementation of a collaborative series of classroom-based...

...collaborations, and commitments to and proof of compliance with the grantor’s requirements as well as state...expertise on the processes needed to successfully implement, manage and close-out a grant. Grant professionals...reports are submitted and project evaluations are completed. To enter and advance the profession, you can ....S. Bureau of Labor and Statistics shows that employment of grant writers is expected to grow by 16% between...grant by another state. With the blessing of my employer, I took on my first consulting job, and I began...mentoring. How do I become a grant professional? Employment opportunities for those with expertise in grant...application writing and development, project implementation, program evaluation and grant management.  ...

...enChIP system enables us to express the CRISPR complex much easier in various cells, including primary...technologies consisting of iChIP and enChIP are recent examples. We also developed the inducible translocation...interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences. Thus, we've made ...system of journals and grant applications, for example. However, at the same time, it's fun to be involved...professional activities because I can contribute by, for example, helping to improve the papers submitted to journals...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These ...develop new technologies, hopefully they'll be completely different from what we are now doing in my lab...